| Sodium selenite reduces hemoglobin-induced venular leakage in the rat mesentery. | |
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MedLine Citation:
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PMID: 12388216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Modified Hbs are being developed as "blood substitutes," but intravascular injection of diaspirin cross-linked Hb (DBBF-Hb) can produce venular leakage. Hb toxicity may arise from reactive oxygen species, so the antioxidant sodium selenite (Na(2)SeO(3)) was used in an attempt to reduce leak formation. In anesthetized Sprague-Dawley rats, one-half of which received 2 x 10(-6) g/ml Na(2)SeO(3) in their drinking water for 3 wk, the mesenteric microvasculature was perfused with 2 mg/ml DBBF-Hb (N = 8) for 10 min. Controls (N = 7) received saline. This was followed by perfusion with FITC-albumin for 3 min, fixation, and microscopic examination. In rats given DBBF-Hb, Na(2)SeO(3) significantly reduced leak number, leak area, and mast cell degranulation. Venular leakage was also reduced in rats that only received Na(2)SeO(3) locally during DBBF-Hb perfusion. However, Na(2)SeO(3) did not affect animals receiving cyanomet-DBBF-Hb instead of DBBF-Hb and significantly increased leak number and mast cell degranulation in animals receiving saline. In vitro, Na(2)SeO(3) reduced the oxidation rate of DBBF-Hb while in the presence of oxidants. These results suggest that Na(2)SeO(3) reduces DBBF-Hb-induced microvascular leakage partly by retarding the oxidation of its heme iron. |
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Authors:
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Ann L Baldwin; Elizabeth B Wiley; Amber G Summers; Abdu I Alayash |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2002-09-26 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 284 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2002-12-17 Completed Date: 2003-01-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H81-91 Citation Subset: IM |
Affiliation:
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Department of Physiology, College of Medicine, University of Arizona, Tucson 85724-5051, USA. abaldwin@u.arizona.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspirin / analogs & derivatives*, metabolism, pharmacology Capillary Permeability / drug effects* Cell Degranulation / physiology Hemoglobins / metabolism, pharmacology* Male Mast Cells / physiology Methemoglobin / analogs & derivatives, pharmacology Oxidation-Reduction / drug effects Oxygen / metabolism Rats Rats, Sprague-Dawley Sodium Selenite / pharmacology* Splanchnic Circulation / drug effects* Venules / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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HL-53047/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 0/diaspirin-cross-linked cyanomethemoglobin; 0/diaspirin-cross-linked hemoglobin; 10102-18-8/Sodium Selenite; 50-78-2/Aspirin; 7782-44-7/Oxygen; 9008-37-1/Methemoglobin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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