| Sodium salicylate inhibits proliferation and induces G1 cell cycle arrest in human pancreatic cancer cell lines. | |
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MedLine Citation:
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PMID: 10631359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mutations most common in pancreatic cancer decrease the ability to control G1 to S cell cycle progression and cellular proliferation. In colorectal cancer cells, nonsteroidal anti-inflammatory drugs inhibit proliferation and induce cell cycle arrest. We examined whether sodium salicylate, an aspirin metabolite, could inhibit proliferation in human pancreatic cancer cell lines (BxPC3 and Panc-1). Quiescent cells were treated with medium containing 10% fetal calf serum, with or without salicylate. Cellular proliferation was measured by MTT assay and bromodeoxyuridine incorporation. The fractions of cells in G0/G1, S, and G2/M phases of the cell cycle were quantitated by fluorescence-activated cell sorting. Results were compared between groups by two-tailed t test. Cyclin D1 expression was determined by Western blot analysis and prostaglandin E2 expression by enzyme-linked immunosorbent assay. Serum-starved cells failed to proliferate, with most arrested in the G1 phase. Salicylate significantly inhibited serum-induced progression from G1 to S phase, cellular proliferation, and the expression of cyclin D1. The concentrations at which 50% of serum-induced proliferation was inhibited were 1.2 mmol/L (Panc-1) and 1.7 mmol/L (BxPC3). The antiproliferative effect of sodium salicylate was not explained by inhibition of prostaglandin E2 production. This study provides further evidence in a noncolorectal cancer model for the antineoplastic effects of nonsteroidal anti-inflammatory drugs. |
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Authors:
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R A Perugini; T P McDade; F J Vittimberga; A J Duffy; M P Callery |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract Volume: 4 ISSN: 1091-255X ISO Abbreviation: J. Gastrointest. Surg. Publication Date: 2000 Jan-Feb |
Date Detail:
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Created Date: 2000-05-19 Completed Date: 2000-05-19 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9706084 Medline TA: J Gastrointest Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 24-32, discussion 32-3 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0333, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
pathology Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Aspirin / pharmacology* Blotting, Western Cell Division / drug effects Cell Separation Cyclin D1 / metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry G1 Phase / drug effects Humans Mutation Pancreatic Neoplasms / genetics, pathology* S Phase / drug effects Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 136601-57-5/Cyclin D1; 50-78-2/Aspirin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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