Document Detail


Sodium salicylate inhibits proliferation and induces G1 cell cycle arrest in human pancreatic cancer cell lines.
MedLine Citation:
PMID:  10631359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mutations most common in pancreatic cancer decrease the ability to control G1 to S cell cycle progression and cellular proliferation. In colorectal cancer cells, nonsteroidal anti-inflammatory drugs inhibit proliferation and induce cell cycle arrest. We examined whether sodium salicylate, an aspirin metabolite, could inhibit proliferation in human pancreatic cancer cell lines (BxPC3 and Panc-1). Quiescent cells were treated with medium containing 10% fetal calf serum, with or without salicylate. Cellular proliferation was measured by MTT assay and bromodeoxyuridine incorporation. The fractions of cells in G0/G1, S, and G2/M phases of the cell cycle were quantitated by fluorescence-activated cell sorting. Results were compared between groups by two-tailed t test. Cyclin D1 expression was determined by Western blot analysis and prostaglandin E2 expression by enzyme-linked immunosorbent assay. Serum-starved cells failed to proliferate, with most arrested in the G1 phase. Salicylate significantly inhibited serum-induced progression from G1 to S phase, cellular proliferation, and the expression of cyclin D1. The concentrations at which 50% of serum-induced proliferation was inhibited were 1.2 mmol/L (Panc-1) and 1.7 mmol/L (BxPC3). The antiproliferative effect of sodium salicylate was not explained by inhibition of prostaglandin E2 production. This study provides further evidence in a noncolorectal cancer model for the antineoplastic effects of nonsteroidal anti-inflammatory drugs.
Authors:
R A Perugini; T P McDade; F J Vittimberga; A J Duffy; M P Callery
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract     Volume:  4     ISSN:  1091-255X     ISO Abbreviation:  J. Gastrointest. Surg.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-05-19     Completed Date:  2000-05-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9706084     Medline TA:  J Gastrointest Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24-32, discussion 32-3     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0333, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics,  pathology
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Aspirin / pharmacology*
Blotting, Western
Cell Division / drug effects
Cell Separation
Cyclin D1 / metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
G1 Phase / drug effects
Humans
Mutation
Pancreatic Neoplasms / genetics,  pathology*
S Phase / drug effects
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 136601-57-5/Cyclin D1; 50-78-2/Aspirin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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