| Sodium pyruvate protects against H(2)O(2) mediated apoptosis in human neuroblastoma cell line-SK-N-MC. | |
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MedLine Citation:
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PMID: 14599660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Free radicals are involved in neuronal damage. The present study was aimed to investigate the protective effect of sodium pyruvate-a free radical scavenger against hydrogen peroxide (H(2)O(2)) induced apoptosis in human neuroblastoma cell line-SK-N-MC. On exposure to H(2)O(2) (0.025 mM) cells exhibited apoptosis within 24 h, demonstrating a high caspase 3 activity by 3 h followed by cleavage of PARP that was maximum at 24 h. A break down in the mitochondrial membrane potential was observed 3 h onwards. Sodium pyruvate protected cells significantly (P<0.05) against apoptosis in a dose dependent manner as assessed for cell viability by dye exclusion method and apoptosis by TUNEL. Sodium pyruvate significantly inhibited caspase 3 activity, cleavage of PARP and breakdown of mitochondrial membrane potential. These data suggest that sodium pyruvate protects neuronal damage caused by H(2)O(2). |
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Authors:
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Jayashree C Jagtap; Anmol Chandele; B A Chopde; Padma Shastry |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chemical neuroanatomy Volume: 26 ISSN: 0891-0618 ISO Abbreviation: J. Chem. Neuroanat. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-11-05 Completed Date: 2004-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8902615 Medline TA: J Chem Neuroanat Country: Netherlands |
Other Details:
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Languages: eng Pagination: 109-18 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, National Center for Cell Science, Government of India An Autonomous Institution, Pune University Campus, Ganeshkhind, 411007 Pune, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants* Apoptosis / drug effects* Blotting, Western Brain Neoplasms / pathology* Caspase 3 Caspases / biosynthesis Cell Line, Tumor Cell Survival Colorimetry DNA Fragmentation Electrophoresis, Polyacrylamide Gel Flow Cytometry Humans Hydrogen Peroxide / antagonists & inhibitors*, toxicity* In Situ Nick-End Labeling Membrane Potentials / physiology Neuroblastoma / pathology* Neuroprotective Agents* Oxidants / toxicity* Pyruvic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Neuroprotective Agents; 0/Oxidants; 127-17-3/Pyruvic Acid; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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