Document Detail

Sodium and proton transport in Mycoplasma gallisepticum.
MedLine Citation:
PMID:  4030695     Owner:  NLM     Status:  MEDLINE    
When washed cells of Mycoplasma gallisepticum were incubated at 37 degrees C in 250 mM 22NaCl, the intracellular Na+ increased, and the K+ decreased. The addition of glucose to these Na+-loaded cells caused Na+ efflux and K+ uptake (both ions moving against concentration gradients). This effect of glucose was blocked by the ATPase inhibitor dicyclohexylcarbodiimide, which prevents the generation of a proton motive force in these cells. In additional experiments, Na+ extrusion was studied by diluting the 22Na+-loaded cells into Na+-free media and following the loss of 22Na+ from the cells. Glucose stimulated 22Na+ extrusion in such cells by a dicyclohexylcarbodiimide-sensitive mechanism. Proton movement was studied by measuring the pH gradient across the cell membrane with the 9-aminoacridine fluorescence technique. Glucose addition to cells preincubated with cations other than Na+ resulted in cell alkalinization (which was prevented by dicyclohexylcarbodiimide). This observation is consistent with the operation of a proton-extruding ATPase. When glucose was added to Na+-loaded cells and diluted into Na+-free media, intracellular acidification was observed, followed several minutes later by a dicyclohexylcarbodiimide-sensitive alkalinization process. The initial acidification was probably due to the operation of an Na+-H+ antiport, since Na+ exit was occurring simultaneously with H+ entry. When Na+-loaded cells were diluted into Na+-containing media, the subsequent addition of glucose resulted in a weak acidification, presumably due to H+ entry in exchange for Na+ (driven by the ATPase) plus a continuous passive influx of Na+. All of the data presented are consistent with the combined operation of an ATP-driven proton pump and an Na+ -H+ exchange reaction.
C Linker; T H Wilson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  163     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-10-23     Completed Date:  1985-10-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1250-7     Citation Subset:  IM    
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MeSH Terms
Biological Transport, Active
Chlorides / metabolism
Dicyclohexylcarbodiimide / pharmacology
Hydrogen-Ion Concentration
Mycoplasma / drug effects,  growth & development,  metabolism*
Potassium / metabolism
Rubidium / metabolism
Sodium / metabolism*
Tetraethylammonium Compounds / metabolism
Grant Support
Reg. No./Substance:
0/Chlorides; 0/Tetraethylammonium Compounds; 538-75-0/Dicyclohexylcarbodiimide; 66-40-0/Tetraethylammonium; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium

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