Document Detail


Sodium nitroprusside inhibition of parathyroid hormone release is not mediated through cyclic GMP.
MedLine Citation:
PMID:  6118808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sodium nitroprusside effected a rapid, dose-dependent increase in intracellular cGMP accumulation in freshly dispersed bovine parathyroid cells. The effect was half-maximal between 10(-4) and 3 X 10(-4)M, maximal at 3 X 10(-3)M nitroprusside and could be amplified (approximately 50%) by the addition of methylisobutylxanthine (4 X 10(-4)M). The dose-response characteristics were similar to those previously described for the inhibition of cAMP accumulation and PTH release by this agent. Neither dibutyryl cGMP (10(-3)M) nor 8'-bromo-cGMP (10(-3)M) mimicked the inhibitory effect of nitroprusside on cAMP accumulation or PTH release. Dose-dependent stimulation of guanylate cyclase was found in a particulate preparation of parathyroid cells; activity was maximal at 10(-4)M nitroprusside while higher concentrations appeared to inhibit the enzyme. Nitroprusside significantly reduced both (-)isoproterenol and guanine nucleotide-stimulated adenylate cyclase activity in the particulate preparation with maximum inhibition between 10(-3)-10(-2)M. cGMP concentrations as high as 10(-4)M did not affect agonist-stimulated cAMP synthesis. Thus, although the kinetic and dose-response characteristics of the nitroprusside effect on cGMP suggest a linkage to its previously described effects on cAMP and PTH secretion, no direct evidence was found to indicate a causal relationship between the two. Rather it would appear that the effects on the adenylate and guanylate cyclase enzymes occur in parallel, possibly the result of some common primary perturbation of cellular physiology.
Authors:
D G Gardner; E M Brown; G D Aurbach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  30     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-22     Completed Date:  1982-02-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1179-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Animals
Cations, Divalent / pharmacology
Cattle
Cyclic AMP / metabolism
Cyclic GMP / analogs & derivatives,  metabolism*
Dose-Response Relationship, Drug
Ferricyanides / pharmacology*
Guanylate Cyclase / metabolism
Nitroprusside / pharmacology*
Parathyroid Glands / drug effects,  metabolism*
Parathyroid Hormone / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Cations, Divalent; 0/Ferricyanides; 0/Parathyroid Hormone; 15078-28-1/Nitroprusside; 60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP; EC 4.6.1.1/Adenylate Cyclase; EC 4.6.1.2/Guanylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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