Document Detail

Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  18647484     Owner:  NLM     Status:  MEDLINE    
Nitric oxide is known to be a messenger in animals and plants. It may act either as a pro-oxidant or antioxidant. In the present work, the yeast Saccharomyces cerevisiae was treated under aerobic conditions with the nitric oxide donor, sodium nitroprusside (SNP), at concentrations of 1, 5 and 10 mM. The activities of antioxidant enzymes as well as concentrations of protein carbonyls and cellular thiols were measured. Yeast incubation with SNP increased the activities of catalase and superoxide dismutase. Cycloheximide, an inhibitor of translation, blocked SNP-induced catalase activation, but not SOD activation. Incubation with SNP increased the activity of peroxisomal catalase, whereas cytosolic catalase was not affected. SNP treatment inactivated aconitase in a dose-dependent manner. Surprisingly, in cells incubated with 1 mM SNP, the levels of low-molecular weight thiols were significantly higher, whereas the concentrations of protein carbonyl groups were lower than those in untreated cells. The incubation of yeast cells either with decomposed SNP or with SNP under anaerobic conditions did not result in SOD and catalase activation. It is suggested, that under aerobic conditions, the SNP effects are connected with induction of mild oxidative/nitrosative stress.
Oleh V Lushchak; Volodymyr I Lushchak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  13     ISSN:  1743-2928     ISO Abbreviation:  Redox Rep.     Publication Date:  2008  
Date Detail:
Created Date:  2008-07-23     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  144-52     Citation Subset:  IM    
Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.
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MeSH Terms
Aconitate Hydratase / metabolism
Catalase / metabolism
Cycloheximide / pharmacology
Cytosol / drug effects,  metabolism
Dose-Response Relationship, Drug
Nitric Oxide Donors / pharmacology
Nitroprusside / pharmacology*
Oxidative Stress / drug effects*
Peroxisomes / drug effects,  metabolism
Protein Synthesis Inhibitors / pharmacology
Saccharomyces cerevisiae / drug effects*,  metabolism*
Saccharomyces cerevisiae Proteins / metabolism
Sulfhydryl Compounds / metabolism
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Nitric Oxide Donors; 0/Protein Synthesis Inhibitors; 0/Saccharomyces cerevisiae Proteins; 0/Sulfhydryl Compounds; 15078-28-1/Nitroprusside; 66-81-9/Cycloheximide; EC; EC Dismutase; EC Hydratase

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