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Sodium-iodide symporter positive cells after intracellular uptake of 99mTc versus α-emitter 211At. Reduction of clonogenic survival and characterization of DNA damage.
MedLine Citation:
PMID:  23037134     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Purpose: We evaluated the DNA damaging potential of Auger electrons emitted in the decay of 99mTc compared to α-particles of 211At. Material and methods: The impact of 99mTc and 211At was monitored in a NIS-expressing rat thyroid cell model PCCl3 with varying, yet defined intra- and extracellular radionuclide distribution (using ± perchlorate). The radiotoxicity of 99mTc and 211At was studied by the comet assay under neutral and alkaline conditions and colony formation. Results: In the presence of perchlorate, the radioactivity yielding 37% cellular survival, A37, was estimated to be (0.27±0.02) MBq/ml and (450±30) MBq/ml for 211At and 99mTc, respectively. In absence of perchlorate, cellular radiotracer uptake was similar for both radionuclides (2.2%, 2.7%), yet the A37 was reduced by 82% for the α-emitter and by 95% for 99mTc. Cellular dose increased by a factor of 5 (211At) and 38 (99mTc). Comet assays revealed an increased DNA damage after intracellular uptake of both radiotracers. Conclusions: The data indicate damage to the cell to occur from absorbed dose without recognizable contribution from intracellular heterogeneity of radionuclide distribution. Comet assay under alkaline and neutral conditions did not reveal any shift to more complex DNA damage after radionuclide uptake. Cellular uptake of 99mTc and 211At increased cellular dose and reduced clonogenic survival.
Authors:
J Kotzerke; M Wendisch; R Freudenberg; R Runge; L Oehme; G J Meyer; L A Kunz-Schughart; G Wunderlich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nuklearmedizin. Nuclear medicine     Volume:  51     ISSN:  0029-5566     ISO Abbreviation:  Nuklearmedizin     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7609387     Medline TA:  Nuklearmedizin     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  170-8     Citation Subset:  IM    
Affiliation:
Prof. Dr. med. Jörg Kotzerke, Klinik und Poliklinik für Nuklearmedizin, Universitätsklinikum Dresden, Fetscherstr. 74, 01307 Dresden, Germany, Tel. +49/(0)351/4584160, Fax +49/(0)351/4585347, E-mail: joerg.kotzerke@mailbox.tu-dresden.de.
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