Document Detail


Sodium iodide symporter and pendrin expression in human thyroid tissues.
MedLine Citation:
PMID:  11575851     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thyroid cells synthesize thyroid hormones through a multistep process during which iodide is transported through the basolateral and the apical membranes of thyrocytes. Two genes that participate in these transports and the corresponding proteins, namely sodium iodide symporter (NIS) and pendrin, the product of the Pendred syndrome gene, have recently been characterized. We studied NIS and pendrin expression at the mRNA and protein levels by a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method and by single and double immunostaining in normal and pathological human thyroid tissues. In normal tissue, NIS and pendrin were detected in about 20% and 40%-60% of thyrocytes, respectively. The number of NIS- and pendrin-positive cells was much higher in hyperfunctioning tissue from Graves' disease or toxic adenoma. In hypofunctioning adenomas and carcinomas, the number of NIS- and pendrin-positive cells was low or nonexistent. Three types of follicular cells were observed in positive tissues: NIS-negative/pendrin-negative cells, NIS-positive/pendrin-positive cells, and NIS-negative/pendrin-positive cells. The first two types of cells appear to be resting and active cells, respectively, but the functional status of NIS-negative/pendrin-positive thyrocytes remains to be determined.
Authors:
C Mian; L Lacroix; L Alzieu; M Nocera; M Talbot; J M Bidart; M Schlumberger; B Caillou
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thyroid : official journal of the American Thyroid Association     Volume:  11     ISSN:  1050-7256     ISO Abbreviation:  Thyroid     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-28     Completed Date:  2002-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104317     Medline TA:  Thyroid     Country:  United States    
Other Details:
Languages:  eng     Pagination:  825-30     Citation Subset:  IM    
Affiliation:
Department of Clinical Biology, Institut Gustave-Roussy, Villejuif, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma, Follicular / metabolism
Adult
Carcinoma, Papillary / metabolism
Carrier Proteins / genetics,  metabolism*
Female
Graves Disease / metabolism
Humans
Male
Membrane Transport Proteins*
Middle Aged
RNA, Messenger / metabolism
Reference Values
Symporters / genetics,  metabolism*
Thyroid Gland / metabolism*
Thyroid Neoplasms / metabolism
Thyroid Nodule / metabolism
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Transport Proteins; 0/RNA, Messenger; 0/SLC26A4 protein, human; 0/Symporters; 0/sodium-iodide symporter

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A high-sensitivity enzyme-linked immunosorbent assay for serum thyroglobulin.
Next Document:  Expression of intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and homing fac...