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Sodium hydrosulfide improves the protective potential of the cardioplegic histidine buffer solution.
MedLine Citation:
PMID:  21185822     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Since H(2)S has an emerging role as a cardioprotector, we hypothesized that NaHS addition to the new cardioplegic histidine buffer solution (HBS) could improve its cardioprotective potential. Male Wistar-Han rat hearts were divided in 4 groups: i) control, ii) perfusion control (perfusion only), iii) 6h ischemia in HBS or in a modified-HBS with 100μM of NaHS, a H(2)S donor, (HBSM) and iv) as iii followed by 30min reperfusion. During ischemia, aliquots of the cardioplegic solution were collected for NMR analysis. Heart mitochondria respiration and transmembrane potential were measured after ischemia or after ischemia followed by reperfusion. Proteins involved in the apoptotic signaling pathway were also quantified in both mitochondrial and tissue samples. Cardiac mechanic performance was evaluated by measuring the heart rate and the left ventricular pressure. In HBSM-preserved hearts, a) glucose consumption increased as well as lactate and alanine production during ischemia, b) heart mitochondria presented an improved phosphorylative efficiency, including decreased phosphorylative lag phase for complex I and complex II substrates, c) mitochondrial and tissue p53, Bax and caspase-9 were lower and d) there was a more positive atrial chronotropic response than in HBS-preserved hearts. We concluded that the addition of NaHS to HBS enhances glycolysis during ischemia, decreases mitochondrial dysfunction, especially by preserving the phosphorylative system, prevents apoptosis and during ischemia/reperfusion.
Authors:
Marco G Alves; Ana F Soares; Rui A Carvalho; Paulo J Oliveira
Publication Detail:
Type:  Journal Article     Date:  2010-12-24
Journal Detail:
Title:  European journal of pharmacology     Volume:  654     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-01-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  60-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Center for Neurosciences and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal; Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, 3004-517 Coimbra, Portugal.
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