| Sodium fluoride induces apoptosis and alters bcl-2 family protein expression in MC3T3-E1 osteoblastic cells. | |
| | |
MedLine Citation:
|
PMID: 21708129 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Chronic excessive fluoride intake is known to be toxic and can lead to fluorosis and bone pathologies. However, the cellular mechanisms underlying NaF-induced cytotoxicity in osteoblasts are not well understood. The objectives of this study were to determine the effects of fluoride treatment on MC3T3-E1 osteoblastic cell viability, cell cycle analysis, apoptosis and the expression levels of bcl-2 family members: bcl-2 and bax. MC3T3-E1 cells were treated with 10(-5); 5×10(-5); 10(-4); 5×10(-4) and 10(-3)M NaF for up to 48h. NaF was found to reduce cell viability in a temporal and concentration dependent manner and promote apoptosis even at low concentrations (10(-5)M). This increased apoptosis was due to alterations in the expression of both pro-apoptotic bax and anti-apoptotic bcl-2. The net result was a decrease in the bcl-2/bax ratio which was found at both the mRNA and protein levels. Furthermore, we also noted that NaF-induced S-phase arrest during the cell cycle of MC3T3-E1 cells. These data suggest that fluoride-induced osteoblast apoptosis is mediated by direct effects of fluoride on the expression of bcl-2 family members. |
| | |
Authors:
|
Shiyong Yang; Zhi Wang; Colin Farquharson; Rashad Alkasir; Mohammad Zahra; Gaixian Ren; Bo Han |
Related Documents
:
|
22144319 - Use of astrocyte-microglial cocultures to examine the regulatory influence of astrocyte... 11489059 - Chemokines in pathology and medicine. 19228389 - Influence of hfe variants and cellular iron on monocyte chemoattractant protein-1. 17533049 - Adiponectin binds to chemokines via the globular head and modulates interactions betwee... 9365119 - Chemokines in neurological disease models: correlation between chemokine expression pat... 19513749 - Expression of cytokines and chemokines and microvasculature alterations of the tongue f... 14525939 - The presence of nitrite during uva irradiation protects from apoptosis. 20300179 - Immune requirements of post-exposure immunization with modified vaccinia ankara of leth... 18938159 - Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-17 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
College of Veterinary Medicine, China Agricultural University, 100193 Beijing, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and identification of dieldrin-degrading Pseudonocardia sp. strain KSF27 using a soil-char...
Next Document: Common features and differences in the expression of the three genes forming the UGA regulon in Sacc...