| Sodium-dependent L-lactate uptake by bovine intestinal brush border membrane vesicles. | |
| | |
MedLine Citation:
|
PMID: 3235729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In ruminants, intestinal digesta can contain considerable amounts of lactic acid derived from ingestion of lactic acid-containing feed and from production of lactic acid during ruminal digestion of readily fermentable carbohydrates. The aim of the present study was to investigate L-lactate transport across the bovine intestinal brush border membrane. The experiments were performed using isolated brush border membrane vesicles from the midjejunum of heifers. The results demonstrate the existence of Na+-stimulated L-lactate uptake by the brush border membrane vesicles. Acetate, propionate, and butyrate strongly inhibited Na+-dependent L-lactate transport. Acetate caused a 58% inhibition, whereas propionate and butyrate completely inhibited Na+-dependent uptake. Kinetic evaluation of L-lactate uptake in the presence or absence of extravesicular butyrate suggests a competitive inhibition by butyrate. Among the phenolic acids tested in this study only trans-cinnamic acid caused a significant reduction of L-lactate uptake, whereas cumaric acid, ferulic acid, and gallic acid only slightly reduced L-lactate transport. Thus, the L-lactate transporter appears to have some affinity for transcinnamic acid. |
| | |
Authors:
|
S Wolffram; B Grenacher; E Scharrer |
Related Documents
:
|
8953569 - Kainic acid, ampa, and dihydrokainic acid effect on uptake and efflux of d-[3h] asparti... 17189489 - Saccharomyces cerevisiae multidrug resistance transporter qdr2 is implicated in potassi... 16660779 - Amino acid uptake into cultivated mesophyll cells from asparagus officinalis l. 7185269 - Cytotoxic material released from staphylococcus epidermidis. ii. fractionation and some... 15351399 - The anandamide membrane transporter. structure-activity relationships of anandamide and... 9755889 - Cellular uptake mechanism of amino acid ester prodrugs in caco-2/hpept1 cells overexpre... 1519259 - Synthesis of 3 alpha, 7 alpha-dihydroxy-5 beta-cholestan-26-oic acid from 3 alpha,7 alp... 9159889 - Biosynthesis of docosahexaenoic acid in trout hepatocytes proceeds via 24-carbon interm... 9002209 - Nucleic acid based sensors. |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Journal of dairy science Volume: 71 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 1988 Dec |
Date Detail:
|
Created Date: 1989-05-11 Completed Date: 1989-05-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3267-73 Citation Subset: IM |
Affiliation:
|
Institut für Veterinär-Physiologie, Universität Zürich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Transport Cattle / metabolism* Female Intestinal Absorption* Jejunum / metabolism* Lactates / pharmacokinetics* Microvilli / metabolism Sodium / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Lactates; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Growth-enhancing supplements for various species of the genus Bifidobacterium.
Next Document: Iodine concentrations in milk of dairy cattle fed various amounts of iodine as ethylenediamine dihyd...