Document Detail

Sodium butyrate induces pyroglutamyl peptidase I and decreases thyrotropin-releasing hormone receptors in GH3 cells.
MedLine Citation:
PMID:  2564336     Owner:  NLM     Status:  MEDLINE    
The effect of sodium butyrate treatment on TRH-degrading enzymes and TRH receptors in GH3 cells was investigated. The specific activity of pyroglutamyl peptidase I (EC was increased by exposure to sodium butyrate in a time- and concentration-dependent manner, whereas the specific activity of prolyl endopeptidase (EC was unchanged. The maximal effect occurred at a concentration of 1 mM sodium butyrate and 16 h after exposure. The increase was reversible upon removal of sodium butyrate from the cell culture. Cycloheximide totally blocked the stimulation, indicating that the increase was due to new protein synthesis. Sodium butyrate had no effect on pyroglutamyl peptidase I activity in the AtT-20 cell line. [methyl-3H]TRH binding to intact GH3 cells was reduced to 70% of the control value when cells were exposed to 1 mM sodium butyrate for 8 h. A maximal decrease in binding to 40% of the control value occurred after 16 h of exposure. The Kd of [methyl-3H]TRH binding was not changed. Sodium butyrate altered GH3 cell morphology, but the morphological changes occurred after alterations of pyroglutamyl peptidase I activity and [methyl-3H]TRH-binding sites. Other agents known to alter GH3 cell morphology had no effect on pyroglutamyl peptidase I activity. These results indicate that sodium butyrate can in some respects mimic the action of T3 on GH3 cells. Moreover, they provide further evidence that the activity of pyroglutamyl peptidase I, but not prolyl endopeptidase, is subject to regulation in the GH3 cell.
C S Suen; S Wilk
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  124     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-03     Completed Date:  1989-05-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1654-60     Citation Subset:  AIM; IM    
Department of Pharmacology, Mount Sinai School of Medicine of the City University of New York, New York 10029.
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MeSH Terms
Aminopeptidases / metabolism*
Butyrates / pharmacology*
Butyric Acid
Butyric Acids / pharmacology*
Cell Line
Dose-Response Relationship, Drug
Pituitary Neoplasms / enzymology,  metabolism*,  pathology
Pyroglutamyl-Peptidase I / metabolism*
Receptors, Neurotransmitter / drug effects,  metabolism
Receptors, Thyrotropin-Releasing Hormone
Time Factors
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Butyrates; 0/Butyric Acids; 0/Receptors, Neurotransmitter; 0/Receptors, Thyrotropin-Releasing Hormone; 107-92-6/Butyric Acid; EC 3.4.11.-/Aminopeptidases; EC I

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