Document Detail

Sodium butyrate-induced upregulation of p18( INK4C ) gene affects K562 cell G (0)/G (1) arrest and differentiation.
MedLine Citation:
PMID:  18642058     Owner:  NLM     Status:  MEDLINE    
Histone deacetylase inhibitor sodium butyrate (NaBu) can induce G(0)/G(1) arrest and erythroid differentiation in K562 cells, but the molecular mechanisms underlying this process are unclear. Here we show that both p18( INK4C ) mRNA and protein levels were upregulated during K562 cell erythroid differentiation induced by NaBu. Moreover, the NaBu activation of p18( INK4C ) was dependent on the integrity of Sp1 clusters in the promoter. NaBu caused hyperacetylation of histones H3 and H4 on endogenous p18( INK4C ) promoter and enhanced binding of transcription factor Sp1 in vivo. Also, overexpression of p18( INK4C ) in K562 cells resulted in G(0)/G(1) arrest and partial erythroid differentiation. Our results suggested that NaBu-mediated p18( INK4C ) regulation played a role in cell cycle arrest and erythroid differentiation in K562 cells.
Lin Li; Guoping Zhang; Ye Zhang; Jiang Tan; Hui Huang; Baiqu Huang; Jun Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-19
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  319     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2009-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  9-15     Citation Subset:  IM    
Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
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MeSH Terms
Butyrates / pharmacology*
Cell Differentiation / drug effects*
Cyclin-Dependent Kinase Inhibitor p18 / biosynthesis*
Erythroid Cells / cytology,  metabolism
G0 Phase / drug effects*
G1 Phase / drug effects*
Histones / metabolism
K562 Cells
Response Elements
Sp1 Transcription Factor / metabolism
Up-Regulation / drug effects*
Reg. No./Substance:
0/Butyrates; 0/CDKN2C protein, human; 0/Cyclin-Dependent Kinase Inhibitor p18; 0/Histones; 0/Sp1 Transcription Factor

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