| Sodium butyrate increases the effect of the photodynamic therapy: a mechanism that involves modulation of gene expression and differentiation in astrocytoma cells. | |
MedLine Citation:
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PMID: 22710635 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVES: In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated. METHODS: Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important increase by ALA was observed in uroporphyrinogen synthetase gene expression: 1.8- and 52-fold for D54-MG and U373-MG cells, respectively. After irradiation, they showed 16.67 and 28.9 % of mortality in U373-MG and D54-MG, respectively. These mortalities increased to 70.62 and 96.7 % when U373-MG and D54-MG cells, respectively, were exposed 24 h to 8 mM NaBu, before to PpIX induction. NaBu induced expression of caspase-3, caspase-9, and Bcl-2 and increased Bax in U373-MG cells. ALA-induced morphological changes are compatible to differentiation. CONCLUSIONS: Genes and differentiation induced mainly by NaBu improve cell death performed by PDT in astrocytoma cells. These facts prove the synergistic effect of NaBu on cytotoxic damage induced by PDT. |
Authors:
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José Bueno-Carrazco; Violeta Castro-Leyva; Fanny García-Gomez; Mario Solís-Paredes; Eva Ramon-Gallegos; Alfredo Cruz-Orea; Pilar Eguía-Aguilar; Francisco Arenas-Huertero |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-19 |
Journal Detail:
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Title: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery Volume: - ISSN: 1433-0350 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8503227 Medline TA: Childs Nerv Syst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Instituto Tecnológico de Sonora, Avenida Antonio Caso s/n Colonia Villa ITSON, 85120, Sonora, Mexico. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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