Document Detail

Sodium arsenite induces the stress response in the gut and decreases bacterial translocation in a burned mouse model with gut-derived sepsis.
MedLine Citation:
PMID:  10774621     Owner:  NLM     Status:  MEDLINE    
Bacteria translocation from the bowel to systemic organs after burn injury may contribute to or be a cause of sepsis and multiple organ failure. The stress response confers protection under stressful conditions that would otherwise lead to cell damage or death. We investigated whether prior induction of the stress response by sodium arsenite could affect bacterial translocation after thermal injury. HSP-70, a highly stress-inducible protein, was used as a marker for induction of the stress response. Balb/c mice were intravenously injected with 4 mg/kg of sodium arsenite and killed at selected times post-treatment. Other treated mice were then gavaged with 10(10) E. coil or 10(10) 111In-labeled E. coil followed by a 20% burn. Survival was observed for 10 days. Mice gavaged with radiolabeled E. coil were killed 4 h post-burn to determine the effect of HSP-70 induction on microbial translocation in mesenteric lymph nodes (MLN), liver, and spleen. Sodium arsenite-injected mice showed HSP-70 induction in the ileum that increased in a time-dependent manner with peak expression 12 h post-injection. Treated mice showed a significantly higher survival rate (93%) than controls (46%; P < 0.05), and detection of 111In-labeled E. coli was significantly less in the liver and spleen (P < 0.05). These data show that sodium arsenite induced HSP-70 expression in the small intestine. The stress response was associated with significantly increased survival and significant decrease in detection of 111In-labeled E. coil in the liver and spleen in a burned mouse model with gut-derived sepsis.
T Eaves-Pyles; H R Wong; J W Alexander
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  13     ISSN:  1073-2322     ISO Abbreviation:  Shock     Publication Date:  2000  
Date Detail:
Created Date:  2000-06-08     Completed Date:  2000-06-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-9     Citation Subset:  IM    
Department of Surgery, The University of Cincinnati, OH 45267-0558, USA.
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MeSH Terms
Arsenites / pharmacology*
Bacterial Translocation / drug effects,  physiology*
Burns / complications,  physiopathology*
Escherichia coli / isolation & purification,  physiology*
HSP70 Heat-Shock Proteins / biosynthesis
Ileum / microbiology,  physiopathology*
Indium Radioisotopes
Liver / microbiology
Lymph Nodes / microbiology
Mice, Inbred BALB C
Sepsis / complications,  physiopathology*
Sodium Compounds / pharmacology*
Spleen / microbiology
Stress, Physiological
Survival Rate
Grant Support
Reg. No./Substance:
0/Arsenites; 0/HSP70 Heat-Shock Proteins; 0/Indium Radioisotopes; 0/Sodium Compounds; 13768-07-5/sodium arsenite

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