| Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. | |
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MedLine Citation:
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PMID: 20164365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In rodents, severe dietary P restriction increases active phosphate absorption by the intestine. However, it remains unknown if moderate dietary P restriction has a similar effect. Weanling pigs (n = 32; body weight 7.4 +/- 0.55 kg) were used in a 2 x 2 factorial design and fed dietary available P (aP) concentrations of 0.23 or 0.40% and Ca concentrations of 0.58 or 1.00% for 14 d. Diets were formulated on an aP basis instead of a total P basis, because pigs are unable to absorb phytate-P present in corn and soybean meal. Jejunal segments were mounted in modified Ussing chambers for determination of Na(+)-dependent nutrient transport. Intestinal mucosal scrapings were taken for RNA isolation and brush border membrane (BBM) vesicle isolation. Na(+)-dependent phosphate uptake and gene expression of Na-phosphate cotransporter IIb (NaPi-IIb), SGLT-1 (sodium/glucose cotransporter-1), and calbindin D(9k) and protein expression of NaPi-IIb were evaluated. Na(+)-dependent phosphate transport increased (P < 0.05) 46% as dietary aP concentration was decreased. However, increased Na(+)-dependent phosphate uptake was not accompanied by increased NaPi-IIb mRNA expression. Expression of NaPi-IIb protein in the BBM increased (P < 0.01) 84% in pigs fed low-P diets compared with pigs fed adequate-P diets. No dietary Ca effects or aP x Ca interactions were detected for Na-dependent P uptake, mRNA or protein expression of NaPi-IIb, or mRNA expression of calbindin D(9k). These data suggest that restricting dietary aP concentration by only 43% stimulates Na(+)-dependent phosphate uptake and expression of the NaPi-IIb protein in the BBM of the small intestine and through a post-transcriptional mechanism. |
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Authors:
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Kari L Saddoris; James C Fleet; John S Radcliffe |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial Date: 2010-02-17 |
Journal Detail:
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Title: The Journal of nutrition Volume: 140 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-04-30 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 731-6 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis Animal Nutritional Physiological Phenomena Animals Calcium / metabolism* Calcium, Dietary / administration & dosage Diet / veterinary* Female Jejunum / metabolism* Male Phosphates / pharmacokinetics* Phosphorus, Dietary / administration & dosage RNA Processing, Post-Transcriptional* Sodium / metabolism* Sodium-Phosphate Cotransporter Proteins, Type IIb / genetics, metabolism Swine |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK054111-12/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium, Dietary; 0/Phosphates; 0/Phosphorus, Dietary; 0/Sodium-Phosphate Cotransporter Proteins, Type IIb; 7440-23-5/Sodium; 7440-70-2/Calcium |
| Comments/Corrections | |
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