Document Detail


Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells.
MedLine Citation:
PMID:  21036719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The effect of combining sodium butyrate (NaB), a histone deacetylase inhibitor, and 7-hydroxy-staurosporine (UCN-01) on cytotoxicity in human cervical carcinoma cells was evaluated.
MATERIALS AND METHODS: HeLa and CaSki cells were treated using NaB alone or in combination with staurosporine (STS) or its analog UCN-01. Cytotoxicity was determined by flow cytometry and morphological assays. Apoptotic pathways were characterized by Western blotting and immunostaining. CaSki cells were also xenografted into nude mice to assess the in vivo effects of NaB/UCN-01 combination.
RESULTS: Treatment with NaB and STS or UCN-01 resulted in enhanced apoptosis of cancer cells. Apoptosis involved mitochondrial pathways and overexpression of p53 and p73. In concordance, co-treatment modulated some p53/p73 downstream targets such as p21, BAX, BCL-2 and BCL-X(L), leading to increased caspase-3 and poly(ADP-ribose) polymerase cleavage. In vivo, NaB/UCN-01 combination exerted a substantial tumour growth suppression effect compared with single treatment.
CONCLUSION: UCN-01 was shown to be a potentiator of NaB therapy for cervical cancer cells.
Authors:
Anne-Zélie Decrion-Barthod; Matthieu Bosset; Marie-Laure Plissonnier; Antonio Marchini; Magalie Nicolier; Sophie Launay; Jean-Luc Prétet; Jean Rommelaere; Christiane Mougin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2010-12-10     Revised Date:  2013-05-24    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4049-61     Citation Subset:  IM    
Affiliation:
Franche-Comte University, EA 3181, IFR 133, University Hospital, Besançon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Apoptosis / drug effects
Butyrates / administration & dosage,  pharmacology*
Cell Cycle / drug effects
Cell Growth Processes / drug effects
Cell Line, Tumor
Female
HeLa Cells
Human papillomavirus 16 / isolation & purification
Human papillomavirus 18 / isolation & purification
Humans
Mice
Mice, Nude
Papillomavirus Infections / pathology,  virology
Staurosporine / administration & dosage,  analogs & derivatives*,  pharmacology
Uterine Cervical Neoplasms / drug therapy*,  pathology,  virology
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Butyrates; 62996-74-1/Staurosporine; 7BU5H4V94A/7-hydroxystaurosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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