| Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells. | |
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MedLine Citation:
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PMID: 21036719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: The effect of combining sodium butyrate (NaB), a histone deacetylase inhibitor, and 7-hydroxy-staurosporine (UCN-01) on cytotoxicity in human cervical carcinoma cells was evaluated. MATERIALS AND METHODS: HeLa and CaSki cells were treated using NaB alone or in combination with staurosporine (STS) or its analog UCN-01. Cytotoxicity was determined by flow cytometry and morphological assays. Apoptotic pathways were characterized by Western blotting and immunostaining. CaSki cells were also xenografted into nude mice to assess the in vivo effects of NaB/UCN-01 combination. RESULTS: Treatment with NaB and STS or UCN-01 resulted in enhanced apoptosis of cancer cells. Apoptosis involved mitochondrial pathways and overexpression of p53 and p73. In concordance, co-treatment modulated some p53/p73 downstream targets such as p21, BAX, BCL-2 and BCL-X(L), leading to increased caspase-3 and poly(ADP-ribose) polymerase cleavage. In vivo, NaB/UCN-01 combination exerted a substantial tumour growth suppression effect compared with single treatment. CONCLUSION: UCN-01 was shown to be a potentiator of NaB therapy for cervical cancer cells. |
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Authors:
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Anne-Zélie Decrion-Barthod; Matthieu Bosset; Marie-Laure Plissonnier; Antonio Marchini; Magalie Nicolier; Sophie Launay; Jean-Luc Prétet; Jean Rommelaere; Christiane Mougin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-01 Completed Date: 2010-12-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 4049-61 Citation Subset: IM |
Affiliation:
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Franche-Comte University, EA 3181, IFR 133, University Hospital, Besançon, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Combined Chemotherapy Protocols / pharmacology* Apoptosis / drug effects Butyrates / administration & dosage, pharmacology* Cell Cycle / drug effects Cell Growth Processes / drug effects Cell Line, Tumor Female Hela Cells Human papillomavirus 16 / isolation & purification Human papillomavirus 18 / isolation & purification Humans Mice Mice, Nude Papillomavirus Infections / pathology, virology Staurosporine / administration & dosage, analogs & derivatives*, pharmacology Uterine Cervical Neoplasms / drug therapy*, pathology, virology Xenograft Model Antitumor Assays |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 112953-11-4/7-hydroxystaurosporine; 62996-74-1/Staurosporine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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