Document Detail


Sodium 4-phenylbutyrate upregulates ENaC and sodium absorption in T84 cells.
MedLine Citation:
PMID:  17098230     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the short circuit current did not display any amiloride-sensitive Na(+) absorption and was wholly representative of Cl(-) secretion. However, when T84 cells were grown in the presence of 5 mM 4-PBA, a gradual appearance of amiloride-sensitive Na(+) channel (ENaC) activity was observed that reached a plateau after 24 h. Quantitative RT-PCR and Western blot studies of ENaC subunit expression indicated that 4-PBA stimulated alpha and gamma subunits. Trichostatin A, an inhibitor of histone deacetylase, mimicked the effects of 4-PBA, suggesting that 4-PBA affects ENaC expression by inhibiting deacetylases. 4-PBA had no effect on ENaC expression in airway epithelial cells indicating tissue-specific effect. We conclude that butyrate plays an important role in regulating colonic Na(+) absorption by increasing ENaC transcription and activity.
Authors:
Claudiu Iordache; Marek Duszyk
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-21
Journal Detail:
Title:  Experimental cell research     Volume:  313     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-12     Completed Date:  2007-03-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-11     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Alberta, 7-46 Med Sci Bldg., Edmonton, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Butyrates / pharmacology
Cells, Cultured
Epithelial Sodium Channel / metabolism*
Humans
Intestinal Absorption / drug effects*
Phenylbutyrates / pharmacology*
Sodium / metabolism*
Up-Regulation
Chemical
Reg. No./Substance:
0/Butyrates; 0/Epithelial Sodium Channel; 0/Phenylbutyrates; 1821-12-1/4-phenylbutyric acid; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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