| Social isolation attenuates rat forebrain 5-HT release induced by KCI stimulation and exposure to a novel environment. | |
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MedLine Citation:
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PMID: 11224190 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The technique of microdialysis was employed to investigate in vivo 5-hydroxytryptamine (5-HT) release in isolation-reared rats compared with socially reared rats. Two methods were employed to stimulate 5-HT release: local KCI injection into the frontal cortex of chloral hydrate anaesthetized rats, and the exposure of freely moving rats to a novel environment (the elevated x-maze). Microdialysis probes were implanted into the frontal cortex in the case of KCI stimulation and the ventral hippocampus in the case of exposure to the novel environment, and perfused with artificial CSF (1µl/min). Dialysis samples were collected every 20min and analysed for 5-HT and 5-hydroxyindole acetic acid (5-HIAA) by HPLC with electrochemical detection. Both KCI injection (1µl, 100mM) and a 20min period on the elevated x-maze produced a significant increase in extracellular 5-HT in the socially reared rats. Neither the increase in extracellular 5-HT induced by KCI nor the increase on exposure to the elevated x-maze were observed in the isolation-reared rats. Dialysate 5-HIAA was not affected in socially reared or isolation-reared rats, in either protocol. These results suggest that isolation-reared rats have a reduced presynaptic neuronal function to release 5-HT. |
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Authors:
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M.J. Bickerdike; I.K. Wright; C.A. Marsden |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Behavioural pharmacology Volume: 4 ISSN: 1473-5849 ISO Abbreviation: Behav Pharmacol Publication Date: 1993 Jun |
Date Detail:
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Created Date: 2001-Feb-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9013016 Medline TA: Behav Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: 231-236 Citation Subset: - |
Affiliation:
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Department of Physiology & Pharmacology, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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