Document Detail


Snail-dependent and -independent epithelial-mesenchymal transition in oral squamous carcinoma cells.
MedLine Citation:
PMID:  16899764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Disappearance of E-cadherin is a milestone for epithelial-mesenchymal transition (EMT), found both in carcinomas and in some fibrotic diseases. We have studied the mechanisms of EMT in oral squamous cell carcinoma (SCC) cells isolated from primary tumor (43A) and its recurrent tumor (43B). Whereas the cells from primary carcinoma displayed a typical phenotype of squamous epithelial cells including E-cadherin and laminin-332 (laminin-5), cells from recurrent tumor expressed characteristics of dedifferentiated, EMT-experienced tumors. 43B cells expressed E-cadherin repressors ZEB-1/deltaEF1 and especially ZEB-2/SIP1, which therefore appear as candidates for endogenous EMT in these cells. Differences between endogenous and exogenous EMT were assessed by transfecting 43A cells with SNAIL cDNA. SNAIL-transfected cells showed complete EMT phenotype with fibroblastoid appearance, vimentin filaments, E-cadherin/N-cadherin switch, lack of hemidesmosomes and, as a new feature of EMT, lack of laminin-332 synthesis. Upregulation of ZEB-1 and ZEB-2 was evident in these cells, suggesting that SNAIL can regulate these E-cadherin repressors. New monoclonal antibodies against SNAIL showed nuclear immunoreactivity not only in the SNAIL-transfected cells but also in carcinoma cells lacking production of Lm-332 and showing signs of EMT. These results suggest that changes in the epithelial cell differentiation program and EMT in SCC cells can result from the interplay among several E-cadherin repressors; however, SNAIL alone is able to accomplish a complete EMT.
Authors:
Minna Takkunen; Reidar Grenman; Mika Hukkanen; Matti Korhonen; Antonio García de Herreros; Ismo Virtanen
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Publication Detail:
Type:  Journal Article     Date:  2006-08-09
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  54     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-17     Completed Date:  2006-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1263-75     Citation Subset:  IM    
Affiliation:
Institute of Biomedicine/Anatomy, P.O. Box 63 (Haartmaninkatu 8), FI-00014 University of Helsinki, Helsinki, Finland. minna.k.takkunen@helsinki.fi
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MeSH Terms
Descriptor/Qualifier:
Aged
Cadherins / biosynthesis
Carcinoma, Squamous Cell / metabolism,  pathology*
Cell Adhesion
Cell Line, Tumor
Cytoskeletal Proteins / metabolism
Epithelium / pathology
Female
Hemidesmosomes / metabolism
Humans
Mesoderm / pathology
Mouth Neoplasms / metabolism,  pathology*
Neoplasm Recurrence, Local
Transcription Factors / biosynthesis,  genetics,  physiology*
Chemical
Reg. No./Substance:
0/Cadherins; 0/Cytoskeletal Proteins; 0/Transcription Factors; 0/snail family transcription factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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