Document Detail


SmpB: a novel outer membrane protein present in some Brachyspira hyodysenteriae strains.
MedLine Citation:
PMID:  16310325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel outer membrane protein-encoding gene was identified in Brachyspira hyodysenteriae. The predicted protein, SmpB, was encoded by a gene that contains regions of identity with that encoding the previously identified lipoprotein SmpA. However, the majority of the reading frame encoding SmpA and SmpB share no detectable similarity. Analysis of several strains revealed that B. hyodysenteriae harbours either smpA or the newly identified gene smpB, but not both. smpB encodes for a slightly larger protein than smpA, 17.6 and 16.8 kDa, respectively. The predicted proteins share an identical leader sequence and the first 10 amino acids of the mature protein, however, the remainder of the predicted protein sequence shows no similarity. It is hypothesised that smpA and smpB are present on the same area of the chromosome. The proteins are antigenically unique, as antisera raised against a strain of B. hyodysenteriae that expresses SmpA cannot detect SmpB and vice versa. Although the presence of an identical leader peptide suggests identical localisation of SmpA and SmpB, it is not known if the two predicted proteins share similar function.
Authors:
James Holden; George Moutafis; Taghrid Istivan; Peter J Coloe; Peter M Smooker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-28
Journal Detail:
Title:  Veterinary microbiology     Volume:  113     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-13     Completed Date:  2007-04-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  109-16     Citation Subset:  IM    
Affiliation:
School of Applied Sciences, PO Box 71, RMIT University, Bundoora 3083, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / biosynthesis,  immunology
Bacterial Outer Membrane Proteins / biosynthesis,  genetics*,  physiology
Base Sequence
Blotting, Southern / methods
Brachyspira hyodysenteriae / chemistry,  genetics*,  isolation & purification,  physiology
Epitopes
Escherichia coli / genetics
Gene Expression
Immunoblotting / methods
Molecular Sequence Data
Polymerase Chain Reaction / methods
RNA-Binding Proteins / genetics*,  immunology,  physiology
Rabbits
Sequence Alignment
Sequence Analysis, DNA
Spirochaetales Infections / microbiology,  veterinary
Swine
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Epitopes; 0/RNA-Binding Proteins; 0/small protein B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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