Document Detail


Smooth muscle actin and vimentin as markers of testis development in the harbour porpoise (Phocoena phocoena).
MedLine Citation:
PMID:  15379925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Testicular development in the harbour porpoise Phocoena phocoena was examined using animals (n = 192) stranded or by-caught off the coast of England, Wales and Scotland. Classification of animals according to their stage of sexual development was undertaken using gonadal morphology and the distribution of cytoskeletal proteins. Smooth muscle actin (SMA) and vimentin proved particularly useful in this respect; SMA was prominent in the myoid peritubular cells of the adult testis, and two stages of peritubular cell SMA expression could be recognized ('absent' or 'incomplete'). The initial appearance of SMA in peritubular cells was associated with significant increases in body length and body weight (P < 0.001), and occurred during the second year of life. Vimentin, which was prominent in prespermatogonia and spermatogonia, sometimes showed a polarized cytoplasmic distribution. This correlated with a developmental stage at which the seminiferous tubule epithelium becomes populated by germ cells (mean age 1.8 years). Several antibodies were tested for their utility as Sertoli cell markers, but none was found to be specific or useful. Nevertheless, immunohistochemical localization of desmin, GATA-4, Ki67 and androgen receptor was possible despite the poor quality of tissue preservation. This study showed that immunohistochemical classification of these individuals provides a robust basis for the recognition of key physiological stages of sexual development in the male harbour porpoise. This may provide an alternative to the estimation of age, body weight and body length in future analyses aimed at detecting possible adverse effects of environmental pollutants on the reproductive potential of wild marine mammals.
Authors:
W V Holt; J Waller; A Moore; P D Jepson; R Deaville; P M Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of anatomy     Volume:  205     ISSN:  0021-8782     ISO Abbreviation:  J. Anat.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-21     Completed Date:  2004-12-17     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  England    
Other Details:
Languages:  eng     Pagination:  201-11     Citation Subset:  IM    
Affiliation:
Institute of Zoology, Zoological Society of London, Regent's Park, UK. bill.holt@ioz.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Actins / analysis*
Animals
Biological Markers / analysis
Immunohistochemistry / methods
Male
Porpoises / growth & development*
Sexual Maturation / physiology*
Spermatogenesis / physiology
Testis / chemistry,  growth & development*,  physiology
Vimentin / analysis*
Chemical
Reg. No./Substance:
0/Actins; 0/Biological Markers; 0/Vimentin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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