Document Detail

Small unilamellar vesicles are able to fuse with Mycoplasma capricolum cells.
MedLine Citation:
PMID:  1901498     Owner:  NLM     Status:  MEDLINE    
We have investigated the fusion characteristics of intact Mycoplasma capricolum cells and small unilamellar vesicles (SUV). The rate and extent of fusion was monitored continuously by octadecylrhodamine B (R18) fluorescence dequenching assay, as well as by intracellular contents mixing, and by sucrose density gradient analysis. The fusion of SUV with M. capricolum cells was found to be dependent on poly(ethylene glycol) (PEG 8000), divalent cations in the medium, and on the cholesterol content of the lipid vesicles. Maximal levels of fusion were obtained with SUV containing 40 mol% cholesterol in the presence of 5% PEG. The rate and extent of fusion were affected by temperature, pH, osmotic pressure, and SUV/mycoplasma ratio. Under optimal fusion conditions, PEG did not increase the rate of exchange of either cholesterol or phospholipids between M. capricolum cells and SUV. Throughout the fusion process, M. capricolum cells remained intact as measured by the retention of [3H]thymidine-labeled components, and viable. M. capricolum cells were rendered nonfusogenic by treatment with glutaraldehyde (greater than 0.01%) or chlorpromazine (greater than 10 microM). Fusion was partially inhibited by treating the cells with the uncoupler CCCP (5 microM) or proteolytic enzymes, suggesting that a proton gradient across the cell membrane is required for the fusion, and that the cells possess proteinase-sensitive receptors that are responsible for a tighter contact with the lipid vesicles.
M Salman; M Tarshis; S Rottem
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1063     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-16     Completed Date:  1991-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  209-16     Citation Subset:  IM    
Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Cell Fusion* / drug effects
Edetic Acid
Hydrogen-Ion Concentration
Magnesium Chloride
Membrane Lipids / metabolism
Mycoplasma / cytology*,  metabolism
Osmolar Concentration
Polyethylene Glycols / pharmacology
Spectrometry, Fluorescence
Reg. No./Substance:
0/Membrane Lipids; 0/Polyethylene Glycols; 60-00-4/Edetic Acid; 7786-30-3/Magnesium Chloride

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