| Small scale biosynthesis and purification of gram quantities of chorismic acid. | |
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MedLine Citation:
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PMID: 8744423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper details improvements in the biosynthetic method of purification of chorismic acid using Klebsiella pneumoniae 62-1. New growth and accumulation conditions yielded 4 L of accumulation medium containing approximately 0.5 g/L of chorismate from 2 L of bacterial growth. Improvements in the handling and extraction procedures produced yields of approximately 2 g of 75 to 90% chorismic acid. A new recrystallization procedure yielded chorismic acid 97% pure by weight (using epsilon 275 = 2630 M-1 cm-1), and 99.8% pure by enzymatic conversion. These results represent a three- to five-fold increase in yield over average published values. |
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Authors:
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C E Rieger; J L Turnbull |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 26 ISSN: 1082-6068 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-09-26 Completed Date: 1996-09-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 67-76 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry Concordia University, Montreal, Quebec. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chorismic Acid
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isolation & purification*,
metabolism* Crystallization Culture Media, Conditioned Drug Stability Hydrogen-Ion Concentration Klebsiella pneumoniae / growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 617-12-9/Chorismic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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