| Small molecule analysis and imaging of fatty acids in the zebra finch song system using time-of-flight-secondary ion mass spectrometry. | |
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MedLine Citation:
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PMID: 21496023 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acids are central to brain metabolism and signaling, but their distributions within complex brain circuits have been difficult to study. Here we applied an emerging technique, time-of-flight secondary ion mass spectrometry (ToF-SIMS), to image specific fatty acids in a favorable model system for chemical analyses of brain circuits, the zebra finch (Taeniopygia guttata). The zebra finch, a songbird, produces complex learned vocalizations under the control of an interconnected set of discrete, dedicated brain nuclei 'song nuclei'. Using ToF-SIMS, the major song nuclei were visualized by virtue of differences in their content of essential and non-essential fatty acids. Essential fatty acids (arachidonic acid and docosahexaenoic acid) showed distinctive distributions across the song nuclei, and the 18-carbon fatty acids stearate and oleate discriminated the different core and shell subregions of the lateral magnocellular nucleus of the anterior nidopallium. Principal component analysis of the spectral data set provided further evidence of chemical distinctions between the song nuclei. By analyzing the robust nucleus of the arcopallium at three different ages during juvenile song learning, we obtain the first direct evidence of changes in lipid content that correlate with progression of song learning. The results demonstrate the value of ToF-SIMS to study lipids in a favorable model system for probing the function of lipids in brain organization, development and function. |
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Authors:
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Kensey R Amaya; Jonathan V Sweedler; David F Clayton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-05-19 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 118 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-15 Completed Date: 2011-09-16 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 499-511 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry. |
Affiliation:
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Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry / physiology Fatty Acids / metabolism* Fatty Acids, Essential / metabolism Finches / physiology* Image Processing, Computer-Assisted Immunohistochemistry Lipid Metabolism / physiology Male Nerve Net / growth & development, physiology Principal Component Analysis Prosencephalon / metabolism, physiology Spectrometry, Mass, Secondary Ion Vocalization, Animal / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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2 R01 NS051820/NS/NINDS NIH HHS; F31 NS060179-01A1/NS/NINDS NIH HHS; P30 DA018310/DA/NIDA NIH HHS; P30 DA018310-08/DA/NIDA NIH HHS; R01 DE018866/DE/NIDCR NIH HHS; R01 DE018866-05/DE/NIDCR NIH HHS; R01 NS051820-13/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Essential |
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