Document Detail


Small intestinal mucins are secreted in proportion to the settling volume in water of dietary indigestible components in rats.
MedLine Citation:
PMID:  16177208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to clarify the effect of dietary indigestible components on small intestinal mucin secretion. We prepared polystyrene foam (PSF) with different expansion ratios (PSF-30, -60 and -90) in which powders had different settling volumes in water (SV). Rats were fed a purified diet containing 0, 10, 30, or 90 g of PSF-60/kg for 10 d. After 8 h of food deprivation, rats were refed 3 g of their respective diets within 90 min. Small intestinal mucin fractions were prepared, and periodic acid/Schiff-reactive substances and O-linked oligosaccharide chains were determined as mucin markers. Feeding of PSF-60 increased the small intestinal mucin secretion dose dependently (control vs. 30 or 60 g of PSF-60/kg, P < 0.05). When rats were fed either purified diet or diets containing PSF-30, 60, or 90 at 10 g/kg for 7 d, small intestinal mucins were greatly affected by the SV of the respective PSF tested. Rats fed the diet containing PSF-90 with the highest SV had the highest amount of mucins (vs. control, P < 0.05). In some natural dietary fibers, the small intestinal mucins and SV were correlated (r = 0.967, P = 0.002). Finally, rats were fed a purified diet or that diet containing 50 g of PSF-60/kg for 7 d. Then, each dietary group was further divided into 2 groups. After 8 h of food deprivation, rats were refed 3 g of purified or PSF diet. Greater mucins in the small intestine were manifest only in rats previously fed the PSF diet whether they were refed purified or PSF diet (control vs. PSF, P < 0.05). These results suggest that the small intestinal mucins are secreted in proportion to the SV of dietary indigestible components, and chronic ingestion of indigestible components is required for the appearance of enhanced mucin secretion.
Authors:
Hiroki Tanabe; Kimio Sugiyama; Tsukasa Matsuda; Shuhachi Kiriyama; Tatsuya Morita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  135     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-22     Completed Date:  2006-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2431-7     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Dietary Fiber / pharmacology
Digestion
Intestinal Mucosa / metabolism*,  secretion
Intestine, Small / metabolism*,  secretion
Male
Mucins / metabolism*,  secretion
Polystyrenes / pharmacology
Rats
Rats, Wistar
Water / metabolism*
Chemical
Reg. No./Substance:
0/Mucins; 0/Polystyrenes; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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