| Small intestinal disaccharidase activity and ileal villus height are increased in piglets consuming formula containing recombinant human insulin-like growth factor-I. | |
| | |
MedLine Citation:
|
PMID: 9212041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of orally administered IGF-I on intestinal development was assessed in piglets. Cesarean-derived, colostrum-deprived piglets received formula alone or formula containing 65 nM (500 microg/L) of recombinant human IGF-I. IGF-I intake averaged 200 microg/kg/d. On d 7 and 14 postpartum, piglets were killed, organs were removed and weighed, and tissue and blood samples were collected. The small intestine was divided into 13 segments that were weighed and measured. A sample of each segment was fixed in formalin, and the mucosa was scraped for enzyme analyses. Food intake, body and organ weights, intestinal weight, length, protein, DNA and RNA content did not differ between the treatment groups. Serum IGF-I, IGF-II, and IGF-binding protein profiles and tissue IGF-binding protein mRNA expression were also comparable between the treatment groups. In contrast, intestinal enzymes and villus height were increased by oral IGF-I. Lactase was approximately 2-fold higher (p < or = 0.05) in the jejunum and proximal ileum, and sucrase was approximately 50% higher (p < or = 0.05) in the jejunum of IGF-I-treated animals than in controls. Villus height in the terminal ileum was approximately 50% greater in IGF-I-treated animals than in controls (p = 0.03). In conclusion, orally administered IGF-I at 200 microg/kg did not affect whole body or organ growth or serum IGF-I concentrations; however, intestinal disaccharidase activity and ileal villus growth were responsive to orally administered IGF-I, supporting a potential role for milk-borne IGF-I in neonatal intestinal development. |
| | |
Authors:
|
V M Houle; E A Schroeder; J Odle; S M Donovan |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Pediatric research Volume: 42 ISSN: 0031-3998 ISO Abbreviation: Pediatr. Res. Publication Date: 1997 Jul |
Date Detail:
|
Created Date: 1997-09-09 Completed Date: 1997-09-09 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 78-86 Citation Subset: IM |
Affiliation:
|
Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Disaccharidases / metabolism* Gene Expression Humans Ileum / drug effects*, growth & development*, metabolism Insulin-Like Growth Factor Binding Proteins / blood, genetics Insulin-Like Growth Factor I / metabolism, pharmacology* Insulin-Like Growth Factor II / metabolism Intestine, Small / drug effects*, enzymology*, metabolism Liver / metabolism RNA, Messenger / genetics, metabolism Recombinant Proteins / pharmacology Swine |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HD29264/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Insulin-Like Growth Factor Binding Proteins; 0/RNA, Messenger; 0/Recombinant Proteins; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II; EC 3.2.1.-/Disaccharidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Major circadian variations of glucose homeostasis in a patient with Rabson-Mendenhall syndrome and p...
Next Document: L-thyroxine treatment of preterm newborns: clinical and endocrine effects.