| Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells. | |
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MedLine Citation:
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PMID: 23275097 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Enoyl-coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells. |
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Authors:
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Xianqiong Gong; Yueyong Zhu; Jing Dong; Jing Chen; Jia You; Qi Zheng; Zilan Rao; Qianguo Mao; Jiaji Jiang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-30 |
Journal Detail:
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Title: Archives of virology Volume: - ISSN: 1432-8798 ISO Abbreviation: Arch. Virol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Center of Liver Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350004, Fujian, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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