Document Detail

Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.
MedLine Citation:
PMID:  23275097     Owner:  NLM     Status:  Publisher    
Enoyl-coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells.
Xianqiong Gong; Yueyong Zhu; Jing Dong; Jing Chen; Jia You; Qi Zheng; Zilan Rao; Qianguo Mao; Jiaji Jiang
Related Documents :
24755677 - Nf-kappa b modulation is involved in celastrol induced human multiple myeloma cell apop...
25048947 - Thyromimetic actions of tetrabromobisphenol a (tbbpa) in steatotic fao rat hepatoma cells.
23593137 - Calpain and reactive oxygen species targets bax for mitochondrial permeabilisation and ...
23963677 - Nevershed and inflorescence deficient in abscission are differentially required for cel...
8744217 - Effects of media, serum, oviductal cells, and hormones during maturation on bovine embr...
25215287 - Phenotypic switch induced by simulated microgravity on mda-mb-231 breast cancer cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Archives of virology     Volume:  -     ISSN:  1432-8798     ISO Abbreviation:  Arch. Virol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Center of Liver Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350004, Fujian, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Phospholipase A(1): a novel virulence factor in Trypanosoma cruzi.
Next Document:  Assessment of nutrient intakes of picky-eating Chinese preschoolers using a modified food frequency ...