| Small-conductance calcium-activated K+ channels 3 (SK3) regulate blastocyst hatching by control of intracellular calcium concentration. | |
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MedLine Citation:
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PMID: 22416006 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUNDThe present study was designed to investigate the expression of small-conductance calcium-activated K(+) channels 3 (SK3) in preimplantation embryos and to explore their role in the underlying mechanism of blastocyst hatching.METHODSHuman preimplantation embryos were donated by patients who achieved successful pregnancy with in vitro fertilization. Mouse preimplantation embryos in different stages were collected and cultured with or without siRNA cell injection. The expression of SK3 was examined by RT-PCR, quantitative real-time PCR, western blot and immunofluorescence. Functional expression of SK3 was investigated using the patch-clamp technique. [Ca(2+)]i was measured by fluorescent imaging. Embryos were cultured in vitro to investigate the effect of SK3 knockdown or apamin, an SK3 inhibitor, on blastocyst hatching and F-actin formation.RESULTSIn human blastocysts, the level of SK3 expression was significantly lower in blastocysts that failed to hatch than in blastocysts that hatched successfully. In mouse embryos, SK3 mRNA and protein were not found in zygotes, but were detected from the 2-cell stage onward, with the highest levels observed in blastocysts. SK3 was predominately located in the trophectoderm cell membrane of expanded blastocysts. SK3 knockdown in trophectoderm cells not only suppressed the SK3 current, but also reduced [Ca(2+)]i elevation and membrane potential hyperpolarization induced by thapsigargin. Although the formation of expanded blastocysts was not affected, blastocyst hatching and F-actin formation were significantly inhibited after SK3 knockdown in trophectoderm cells.CONCLUSIONSSK3-mediated [Ca(2+)]i elevation and membrane potential hyperpolarization in trophectoderm cells are important for blastocyst hatching, and defects in SK3 expression may contribute to infertility. |
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Authors:
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Yong-Chao Lu; Guo-Lian Ding; Jing Yang; Yan-Ling Zhang; Shuai Shi; Run-Ju Zhang; Dan Zhang; Jie-Xue Pan; Jian-Zhong Sheng; He-Feng Huang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-12 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: - ISSN: 1460-2350 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang 310006, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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