| Small is beautiful: N-trimethyl chitosan-ovalbumin conjugates for microneedle-based transcutaneous immunisation. | |
| | |
MedLine Citation:
|
PMID: 21443959 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
To provoke an immune response, a transcutaneously administered vaccine has to diffuse into the skin, reach the lymph nodes and be taken up by dendritic cells (DCs). To study these three steps we immunised mice transcutaneously (with microneedles), intradermally and intranodally. The effect of the formulation was investigated by formulating ovalbumin (OVA) in three ways with N-trimethyl chitosan (TMC): TMC+OVA mixtures, TMC-OVA conjugates and TMC/OVA nanoparticles. Both the percentage OVA(+) DCs in the lymph node and the resultant immunogenicity (serum IgG titres) were studied. Transcutaneously, the TMC-OVA conjugates induced the highest IgG levels and resulted in more OVA(+) DCs in the lymph nodes after 24h than the other TMC formulations. Intradermally, all TMC-adjuvanted OVA formulations increased IgG titres compared to plain OVA. These formulations formed a depot in the skin, prolonging OVA delivery to the lymph nodes. The prolonged delivery of TMC-adjuvanted OVA to lymph node resident DCs was also observed after intranodal immunisation, but in this case the higher uptake did not correspond with elevated antibody titres compared to plain OVA. In conclusion, after transcutaneous administration, TMC-OVA conjugates are most immunogenic among the tested formulations, likely because they penetrate the skin more easily than nanoparticles and consequently are better delivered to DCs, while they show higher uptake by DCs than TMC+OVA mixtures. |
| | |
Authors:
|
Suzanne M Bal; Bram Slütter; Wim Jiskoot; Joke A Bouwstra |
Related Documents
:
|
21410769 - Expression of apoptosis-inducing factor (aif) in keratoacanthomas and squamous cell car... 15649839 - Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protectio... 21519409 - An update on the role of the sebaceous gland in the pathogenesis of acne. 18350549 - Amp-activated protein kinase regulates lymphocyte responses to metabolic stress but is ... 21034299 - Chronic stress and environmental enrichment as opposite factors affecting the immune re... 12542989 - Immunopharmacology of hypersensitivity reactions to drugs. 20716639 - Urokinase plasminogen activator receptor and/or matrix metalloproteinase-9 inhibition i... 18838169 - Complement c1q chemoattracts human dendritic cells and enhances migration of mature den... 21410769 - Expression of apoptosis-inducing factor (aif) in keratoacanthomas and squamous cell car... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-25 |
Journal Detail:
|
Title: Vaccine Volume: - ISSN: 1873-2518 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406899 Medline TA: Vaccine Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
|
Division of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Therapeutic Trajectory Following Intra-Articular Hyaluronic acid Injection in Knee Osteoarthritis - ...
Next Document: Four year immunogenicity of the RTS,S/AS02(A) malaria vaccine in Mozambican children during a phase ...