| Small-angle neutron scattering by a strongly denatured protein: analysis using random polymer theory. | |
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MedLine Citation:
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PMID: 8994618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Small-angle neutron scattering profiles are presented from phosphoglycerate kinase, in the native form and strongly denatured in 4 M guanidinium chloride (GdnHCl) solution. The data are interpreted using a model in which the excess scattering density associated with the protein is represented as a finite freely jointed chain of spheres. The similarity of the model-derived scattering function to experiment increases asymptotically with the number of spheres. The improvement of the fit obtained with more than approximately 200 spheres (i.e., two residues per sphere) is insignificant. The effects of finite size of the scattering units and of scattering length variation along the polypeptide chain are examined. Improved agreement with experiment is obtained when these effects are taken into account. A method for rapid calculation of the scattering profile of a full, all-atom configuration is examined. It is found that a representation of the chain containing two scattering units per residue, placed at the backbone and side-chain scattering length centroids, reproduces the full, all-atom profile to within 2%. |
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Authors:
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A J Petrescu; V Receveur; P Calmettes; D Durand; M Desmadril; B Roux; J C Smith |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biophysical journal Volume: 72 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-05-27 Completed Date: 1997-05-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 335-42 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Bucharest, Romania. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fourier Analysis Guanidine Guanidines Kinetics Models, Structural* Models, Theoretical* Neutrons Phosphoglycerate Kinase / chemistry* Probability Protein Conformation* Protein Denaturation* Random Allocation Recombinant Proteins / chemistry Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
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0/Guanidines; 0/Recombinant Proteins; 113-00-8/Guanidine; EC 2.7.2.3/Phosphoglycerate Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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