| The small ubiquitin-like modifier (SUMO) is essential in cell cycle regulation in Trypanosoma brucei. | |
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MedLine Citation:
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PMID: 20045687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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SUMO, a reversible post-translational protein modifier, plays important roles in many processes of higher eukaryotic cell life. Although SUMO has been identified in many eukaryotes, SUMO and SUMO system are still unknown in some eukaryotic unicellular organisms, such as Trypanosoma brucei (T. brucei). In this study, only one SUMO homologue (TbSUMO) was identified in T. brucei. Expression of TbSUMO was knocked down by using RNA interference technique in procyclic-form T. brucei. The growth of TbSUMO-deficient cells was significantly inhibited. TbSUMO-deficient cells were arrested in G2/M phase accompanied with an obvious increase of 0N1K cells (zoids), and failed in chromosome segregation. These results indicate that TbSUMO is essential in cell cycle regulation, with one important role in mitosis. Meanwhile, the enrichment of zoids suggests the inhibition of mitosis does not prevent the cell division in procyclic-form T. brucei. HA-tagged TbSUMO was overexpressed in T. brucei and was shown to be localized to the nucleus through the whole cell cycle, further revealing its distinguished functions in nucleus. All these accumulated data imply that a SUMO system essential for regulating cell cycle progression might exist in the procyclic-form T. brucei. |
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Authors:
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Shanhui Liao; Tao Wang; Kai Fan; Xiaoming Tu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-04 |
Journal Detail:
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Title: Experimental cell research Volume: 316 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-03-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 704-15 Citation Subset: IM |
Copyright Information:
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2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antimetabolites / metabolism Bromodeoxyuridine / metabolism Cell Cycle / physiology* Cell Nucleus / metabolism Humans Mitotic Spindle Apparatus / metabolism, ultrastructure Molecular Sequence Data Protein Processing, Post-Translational* Protozoan Proteins / genetics, metabolism* RNA Interference SUMO-1 Protein / genetics, metabolism* Sequence Alignment Sequence Homology, Amino Acid Trypanosoma brucei brucei / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites; 0/Protozoan Proteins; 0/SUMO-1 Protein; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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