Document Detail


Small molecule anticonvulsant agents with potent in vitro neuroprotection.
MedLine Citation:
PMID:  22535312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Severe seizure activity is associated with recurring cycles of excitotoxicity and oxidative stress that result in progressive neuronal damage and death. Intervention to halt these pathological processes is a compelling disease-modifying strategy for the treatment of seizure disorders. In the present study, a core small molecule with anticonvulsant activity has been structurally optimized for neuroprotection. Phenotypic screening of rat hippocampal cultures with nutrient medium depleted of antioxidants was utilized as a disease model. Increased cell death and decreased neuronal viability produced by acute treatment with glutamate or hydrogen peroxide were prevented by our novel molecules. The neuroprotection associated with this chemical series has marked structure activity relationships that focus on modification of the benzylic position of a 2-phenyl-2-hydroxyethyl sulfamide core structure. Complete separation between anticonvulsant activity and neuroprotective action was dependent on substitution at the benzylic carbon. Chiral selectivity was evident in that the S-enantiomer of the benzylic hydroxy group had neither neuroprotective nor anticonvulsant activity, while the R-enantiomer of the lead compound had full neuroprotective action at <40 nM and antiseizure activity in three animal models. These studies indicate that potent, multifunctional neuroprotective anticonvulsants are feasible within a single molecular entity.
Authors:
Douglas E Brenneman; Garry R Smith; Yan Zhang; Yanming Du; Sandeep K Kondaveeti; Michael J Zdilla; Allen B Reitz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-04-26
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  47     ISSN:  1559-1166     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-28     Completed Date:  2013-03-26     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  368-79     Citation Subset:  IM    
Affiliation:
Advanced Neural Dynamics, Inc., Pennsylvania Biotechnology Center, Doylestown, PA 18902, USA. dbrenneman@advneuraldynamics.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticonvulsants / chemistry,  pharmacology*
Caco-2 Cells
Cells, Cultured
Disease Models, Animal
Epilepsy / drug therapy*,  pathology
Hippocampus / cytology,  drug effects
Humans
Mice
Neurons / drug effects*,  pathology
Neuroprotective Agents / chemistry,  pharmacology*
Rats
Sulfonamides / chemistry,  pharmacology*
Grant Support
ID/Acronym/Agency:
R43 NS066537/NS/NINDS NIH HHS; R43NS066537/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Neuroprotective Agents; 0/Sulfonamides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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