Document Detail


Small mammal investigation in spotted fever focus with DNA-barcoding and taxonomic implications on rodents species from Hainan of China.
MedLine Citation:
PMID:  22952689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although mammals are a well-studied group of animals, making accurate field identification of small mammals is still complex because of morphological variation across developmental stages, color variation of pelages, and often damaged osteological and dental characteristics. In 2008, small mammals were collected for an epidemiological study of a spotted fever outbreak in Hainan, China. Ten species of small mammals were identified by morphological characters in the field, most using pelage color characters only. The study is extended here, in order to assess whether DNA barcoding would be suitable as an identification tool in these small mammals. Barcode clusters showed some incongruence with morphospecies, especially for some species of Rattus and Niviventer, so molecular delineation was carried out with an expanded dataset of combined cytochrome b (Cyt-b) and cytochrome c oxidase subunit I (COI) sequences. COI sequences were successfully amplified from 83% of collected mammals, but failed in all specimens of Suncus murinus, which were thus excluded in DNA barcoding analysis. Of note, ten molecular taxonomic units were found from samples of nine morphologically identified species. Accordingly, 11 species of small mammals were present in the investigated areas, including four Rattus species, three Niviventer species, Callosciurus erythraeus, Neohylomys hainanensis, Tupaia belangeri, and Suncus murinus. Based on the results of the phylogenetic and molecular delineation analyses, the systematic status of some rodent species should be redefined. R. rattus hainanicus and R. rattus sladeni are synonyms of R. andamanensis. R. losea from China and Southeast Asia comprises two independent species: R. losea and R. sakeratensis. Finally, the taxonomic status of three putative species of Niviventer should be further confirmed according to morphological, molecular and ecological characters.
Authors:
Liang Lu; Douglas Chesters; Wen Zhang; Guichang Li; Ying Ma; Huailei Ma; Xiuping Song; Haixia Wu; Fengxia Meng; Chaodong Zhu; Qiyong Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-29
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-06     Completed Date:  2013-02-19     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e43479     Citation Subset:  IM    
Affiliation:
Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
China
Cluster Analysis
Cytochromes b / metabolism
DNA Barcoding, Taxonomic / methods*
Ecology
Electron Transport Complex IV / metabolism
Geography
Models, Genetic
Phylogeny
Phylogeography
Rats
Rickettsia Infections / genetics*
Rodentia / genetics*
Species Specificity
Chemical
Reg. No./Substance:
9035-37-4/Cytochromes b; EC 1.9.3.1/Electron Transport Complex IV
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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