Document Detail

Small Demodex populations colonize most parts of the skin of healthy dogs.
MedLine Citation:
PMID:  23331694     Owner:  NLM     Status:  In-Data-Review    
Background - It is unproven that all dogs harbour Demodex mites in their skin. In fact, several microscopic studies have failed to demonstrate mites in healthy dogs. Hypothesis/Objectives - Demodex canis is a normal inhabitant of the skin of most, if not all, dogs. This hypothesis was tested using a sensitive real-time PCR to detect Demodex DNA in the skin of dogs. Animals - One hundred dogs living in a humane society shelter, 20 privately owned and healthy dogs and eight dogs receiving immunosuppressive or antineoplastic therapy. Methods - Hair samples (250-300 hairs with their hair bulbs) were taken from five or 20 skin locations. A real-time PCR that amplifies a 166 bp sequence of the D. canis chitin synthase gene was used. Results - The percentage of positive dogs increased with the number of sampling points. When a large canine population was sampled at five cutaneous locations, 18% of dogs were positive for Demodex DNA. When 20 skin locations were sampled, all dogs tested positive for mite DNA. Our study indicates that Demodex colonization of the skin is present in all dogs, independent of age, sex, breed or coat. Nevertheless, the population of mites in a healthy dog appears to be small. Demodex DNA was amplified from all 20 cutaneous points investigated, without statistically significant differences. Conclusions and clinical importance - Using a real-time PCR technique, Demodex mites, albeit in very low numbers, were found to be normal inhabitants of haired areas of the skin of healthy dogs.
Iván Ravera; Laura Altet; Olga Francino; Armand Sánchez; Wendy Roldán; Sergio Villanueva; Mar Bardagí; Lluís Ferrer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary dermatology     Volume:  24     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  168-e37     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD.
Department of Animal Medicine and Surgery Servei Veterinari de Genètica Molecular, Veterinary School, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain.
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