Document Detail


Slugs: potential novel vectors of Escherichia coli O157.
MedLine Citation:
PMID:  16391036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Field and laboratory studies were performed to determine whether slugs could act as novel vectors for pathogen (e.g., Escherichia coli O157) transfer from animal feces to salad vegetables. Escherichia coli O157 was isolated from 0.21% of field slugs from an Aberdeenshire sheep farm. These isolates carried the verocytotoxin genes (vt1 and vt2) and the attaching and effacing gene (eae), suggesting that they are potentially pathogenic to humans. Strain typing using multilocus variable number tandem repeats analysis showed that slug and sheep isolates were indistinguishable. Laboratory experiments using an E. coli mutant resistant to nalidixic acid showed that the ubiquitous slug species Deroceras reticulatum could carry viable E. coli on its external surface for up to 14 days. Slugs that had been fed E. coli shed viable bacteria in their feces with numbers showing a short but statistically significant linear log decline. Further, it was found that E. coli persisted for up to 3 weeks in excreted slug feces, and hence, we conclude that slugs have the potential to act as novel vectors of E. coli O157.
Authors:
Emma L Sproston; M Macrae; Iain D Ogden; Michael J Wilson; Norval J C Strachan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-04     Completed Date:  2006-03-01     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  144-9     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, Cruickshank Building, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Adhesins, Bacterial / genetics
Animal Husbandry
Animals
Disease Vectors*
Escherichia coli Infections / microbiology,  transmission*
Escherichia coli O157 / classification,  genetics,  isolation & purification*,  pathogenicity
Escherichia coli Proteins / genetics
Feces / microbiology
Gastropoda / microbiology*
Minisatellite Repeats / genetics
Sheep
Shiga Toxin 1 / genetics
Shiga Toxin 2
Shiga Toxins / genetics
Chemical
Reg. No./Substance:
0/Adhesins, Bacterial; 0/Escherichia coli Proteins; 0/Shiga Toxin 1; 0/Shiga Toxin 2; 0/Shiga Toxins; 0/verocytotoxin 2; 147094-99-3/eaeA protein, E coli
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