| Slow axonal transport impairment of cytoskeletal proteins in streptozocin-induced diabetic neuropathy. | |
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MedLine Citation:
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PMID: 2475585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The impairment of slow axonal transport of cytoskeletal proteins was studied in the sciatic nerves of streptozocin-diabetic rats. [35S]Methionine was unilaterally injected into the fourth lumbar ganglion and spinal cord, to label the sensory and motor axons, respectively, and then the polymerized elements of the cytoskeleton and the corresponding soluble proteins were analyzed separately. In addition, the pellet/supernatant ratio for tubulin and actin was also assessed. Our results indicate that the velocity of slow component a (SCa) of axonal transport, particularly that of neurofilaments, was strongly reduced (by 60%) in sensory axons. At the same time, a decreased pellet/supernatant ratio of tubulin, possibly owing to a depolymerization of stable microtubules, was also observed. The transport of slow component b (SCb) of axonal transport was also impaired, but the extent of this impairment could not be precisely evaluated. In contrast, motor axons showed little or no impairment of both SCa and SCb at the time studied, a result suggesting a delayed development of the neuropathy in motor axons. |
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Authors:
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P Macioce; G Filliatreau; B Figliomeni; R Hassig; J Thiéry; L Di Giamberardino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 53 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1989-10-12 Completed Date: 1989-10-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1261-7 Citation Subset: IM |
Affiliation:
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Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axonal Transport* Cytoskeletal Proteins / physiology* Diabetes Mellitus, Experimental / physiopathology* Diabetic Neuropathies / physiopathology* Female Ganglia, Spinal / physiopathology Motor Neurons / physiology Neurons, Afferent / physiology Rats Rats, Inbred Strains Reference Values Sciatic Nerve / physiopathology* |
| Chemical | |
Reg. No./Substance:
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0/Cytoskeletal Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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