Document Detail


Slits affect the timely migration of neural crest cells via Robo receptor.
MedLine Citation:
PMID:  22689303     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Neural crest cells emerge by delamination from the dorsal neural tube and give rise to various components of the peripheral nervous system in vertebrate embryos. These cells change from non-motile into highly motile cells migrating to distant areas before further differentiation. Mechanisms controlling delamination and subsequent migration of neural crest cells are not fully understood. Slit2, a chemorepellant for axonal guidance that repels and stimulates motility of trunk neural crest cells away from the gut has recently been suggested to be a tumor suppressor molecule. The goal of this study was to further investigate the role of Slit2 in trunk neural crest cell migration by constitutive expression in neural crest cells.
RESULTS: We found that Slit gain-of-function significantly impaired neural crest cell migration while Slit loss-of-function favored migration. In addition, we observed that the distribution of key cytoskeletal markers was disrupted in both gain and loss of function instances.
CONCLUSIONS: These findings suggest that Slit molecules might be involved in the processes that allow neural crest cells to begin migrating and transitioning to a mesenchymal type.
Authors:
Dion Giovannone; Michelle Reyes; Rachel Reyes; Lisa Correa; Darwin Martinez; Hannah Ra; Gustavo Gomez; Joshua Kaiser; Le Ma; Mary-Pat Stein; Maria Elena de Bellard
Related Documents :
14732703 - Retinoblastoma susceptibility gene product (prb) and p107 functionally separate the req...
8390033 - Regulation of muscarinic stimulation of norepinephrine release and pi hydrolysis during...
18401443 - Fluorescence intensity and intermittency as tools for following dopamine bioconjugate p...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-23
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  241     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2012-11-28     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1274-88     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics,  physiology
Cell Movement / genetics,  physiology*
Cells, Cultured
Chick Embryo
Chickens
Cytoskeleton / genetics,  metabolism
In Situ Hybridization
Intercellular Signaling Peptides and Proteins / genetics,  metabolism*
Microscopy, Video
Nerve Tissue Proteins / genetics,  metabolism*
Neural Crest / cytology*
Receptors, Immunologic / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Time-Lapse Imaging
Grant Support
ID/Acronym/Agency:
1R15-NS060099-01/NS/NINDS NIH HHS; 1SC2GM086312/GM/NIGMS NIH HHS; R01 NS062047/NS/NINDS NIH HHS; R15 NS060099/NS/NINDS NIH HHS; SC2 GM086312/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Immunologic; 0/Slit homolog 2 protein; 0/roundabout protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Exploring Between the Extremes: Conversion-Dependent Kinetics of Phosphite-Modified Hydroformylation...
Next Document:  Design, fabrication and in vitro evaluation of a novel polymer-hydrogel hybrid scaffold for bone tis...