| Slits affect the timely migration of neural crest cells via Robo receptor. | |
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MedLine Citation:
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PMID: 22689303 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Background: Neural crest cells emerge by delamination from the dorsal neural tube and give rise to various components of the peripheral nervous system in vertebrate embryos. These cells change from non-motile into highly motile cells migrating to distant areas before further differentiation. Mechanisms controlling delamination and subsequent migration of neural crest cells are not fully understood. Slit2, a chemorepellant for axonal guidance that repels and stimulates motility of trunk neural crest cells away from the gut has recently been suggested to be a tumor suppressor molecule. The goal of this study was to further investigate the role of Slit2 in trunk neural crest cell migration by constitutive expression in neural crest cells. Results: We found that Slit gain-of-function significantly impaired neural crest cell migration while Slit loss-of-function favored migration. In addition, we observed that the distribution of key cytoskeletal markers was disrupted in both gain and loss of function instances. Conclusions: These findings suggest that Slit molecules might be involved in the processes that allow neural crest cells to begin migrating and transitioning to a mesenchymal type. |
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Authors:
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Dion Giovannone; Michelle Reyes; Rachel Reyes; Lisa Correa; Darwin Martinez; Hannah Ra; Gustavo Gomez; Joshua Kaiser; Le Ma; Mary-Pat Stein; Maria Elena de Bellard |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-06-23 |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 241 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-18 Completed Date: 2012-11-28 Revised Date: 2013-04-24 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 1274-88 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Biology Department, California State University Northridge, Northridge, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / genetics, physiology Cell Movement / genetics, physiology* Cells, Cultured Chick Embryo Chickens Cytoskeleton / genetics, metabolism In Situ Hybridization Intercellular Signaling Peptides and Proteins / genetics, metabolism* Microscopy, Video Nerve Tissue Proteins / genetics, metabolism* Neural Crest / cytology* Receptors, Immunologic / genetics, metabolism* Reverse Transcriptase Polymerase Chain Reaction Time-Lapse Imaging |
| Grant Support | |
ID/Acronym/Agency:
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1R15-NS060099-01/NS/NINDS NIH HHS; 1SC2GM086312/GM/NIGMS NIH HHS; R01 NS062047/NS/NINDS NIH HHS; R15 NS060099/NS/NINDS NIH HHS; SC2 GM086312/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Intercellular Signaling Peptides and Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Immunologic; 0/Slit homolog 2 protein; 0/roundabout protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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