Document Detail

Slit and Robo control cardiac cell polarity and morphogenesis.
MedLine Citation:
PMID:  16360689     Owner:  NLM     Status:  MEDLINE    
Basic aspects of heart morphogenesis involving migration, cell polarization, tissue alignment, and lumen formation may be conserved between Drosophila and humans, but little is known about the mechanisms that orchestrate the assembly of the heart tube in either organism. The extracellular-matrix molecule Slit and its Robo-family receptors are conserved regulators of axonal guidance. Here, we report a novel role of the Drosophila slit, robo, and robo2 genes in heart morphogenesis. Slit and Robo proteins specifically accumulate at the dorsal midline between the bilateral myocardial progenitors forming a linear tube. Manipulation of Slit localization or its overexpression causes disruption in heart tube alignment and assembly, and slit-deficient hearts show disruptions in cell-polarity marker localization within the myocardium. Similar phenotypes are observed when Robo and Robo2 are manipulated. Rescue experiments suggest that Slit is secreted from the myocardial progenitors and that Robo and Robo2 act in myocardial and pericardial cells, respectively. Genetic interactions suggest a cardiac morphogenesis network involving Slit/Robo, cell-polarity proteins, and other membrane-associated proteins. We conclude that Slit and Robo proteins contribute significantly to Drosophila heart morphogenesis by guiding heart cell alignment and adhesion and/or by inhibiting cell mixing between the bilateral compartments of heart cell progenitors and ensuring proper polarity of the myocardial epithelium.
Li Qian; Jiandong Liu; Rolf Bodmer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Current biology : CB     Volume:  15     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-10-26     Revised Date:  2008-10-15    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2271-8     Citation Subset:  IM    
The Burnham Institute, Center for Neuroscienes and Aging, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
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MeSH Terms
Cell Adhesion / physiology
Cell Polarity / physiology
Drosophila / embryology*,  genetics
Drosophila Proteins / metabolism*
Gene Expression Regulation, Developmental*
Heart / embryology*
Morphogenesis / genetics,  physiology*
Nerve Tissue Proteins / metabolism*
Receptors, Immunologic / metabolism*
Reg. No./Substance:
0/Drosophila Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Immunologic; 0/lea protein, Drosophila; 0/roundabout protein; 0/sli protein, Drosophila

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