Document Detail


Slit and Robo control cardiac cell polarity and morphogenesis.
MedLine Citation:
PMID:  16360689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Basic aspects of heart morphogenesis involving migration, cell polarization, tissue alignment, and lumen formation may be conserved between Drosophila and humans, but little is known about the mechanisms that orchestrate the assembly of the heart tube in either organism. The extracellular-matrix molecule Slit and its Robo-family receptors are conserved regulators of axonal guidance. Here, we report a novel role of the Drosophila slit, robo, and robo2 genes in heart morphogenesis. Slit and Robo proteins specifically accumulate at the dorsal midline between the bilateral myocardial progenitors forming a linear tube. Manipulation of Slit localization or its overexpression causes disruption in heart tube alignment and assembly, and slit-deficient hearts show disruptions in cell-polarity marker localization within the myocardium. Similar phenotypes are observed when Robo and Robo2 are manipulated. Rescue experiments suggest that Slit is secreted from the myocardial progenitors and that Robo and Robo2 act in myocardial and pericardial cells, respectively. Genetic interactions suggest a cardiac morphogenesis network involving Slit/Robo, cell-polarity proteins, and other membrane-associated proteins. We conclude that Slit and Robo proteins contribute significantly to Drosophila heart morphogenesis by guiding heart cell alignment and adhesion and/or by inhibiting cell mixing between the bilateral compartments of heart cell progenitors and ensuring proper polarity of the myocardial epithelium.
Authors:
Li Qian; Jiandong Liu; Rolf Bodmer
Related Documents :
22054419 - Jumping the barrier: ve-cadherin, vegf and other angiogenic modifiers in cancer.
22068159 - The non-canonical notch ligand dlk1 exhibits a novel vascular role as a strong inhibito...
10460679 - Effect of anticoagulation and lavage prior to casting of postmortem material with merco...
23234839 - Nuclear segmentation, condensation and bilateral symmetry in polymorphonuclear leukocyt...
22257379 - The expanding role of α2-3 sialylation for leukocyte trafficking in vivo.
22002459 - Arno regulates vegf dependent tissue responses by stabilizing endothelial vegfr-2 surfa...
19692649 - In vitro and in vivo imaging of antivasculogenesis induced by noggin protein expression...
11735359 - Size effects of realgar particles on apoptosis in a human umbilical vein endothelial ce...
16187009 - Effect of nebivolol and atenolol on brachial artery flow-mediated vasodilation in patie...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Current biology : CB     Volume:  15     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-10-26     Revised Date:  2008-10-15    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2271-8     Citation Subset:  IM    
Affiliation:
The Burnham Institute, Center for Neuroscienes and Aging, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / physiology
Cell Polarity / physiology
Drosophila / embryology*,  genetics
Drosophila Proteins / metabolism*
Gene Expression Regulation, Developmental*
Heart / embryology*
Immunohistochemistry
Morphogenesis / genetics,  physiology*
Nerve Tissue Proteins / metabolism*
Receptors, Immunologic / metabolism*
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Nerve Tissue Proteins; 0/Receptors, Immunologic; 0/lea protein, Drosophila; 0/roundabout protein; 0/sli protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Control of Shugoshin function during fission-yeast meiosis.
Next Document:  Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid b...