Document Detail


Sliding contact loading enhances the tensile properties of mesenchymal stem cell-seeded hydrogels.
MedLine Citation:
PMID:  22791371     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The primary goal of cartilage tissue engineering is to recapitulate the functional properties and structural features of native articular cartilage. While there has been some success in generating near-native compressive properties, the tensile properties of cell-seeded constructs remain poor, and key features of cartilage, including inhomogeneity and anisotropy, are generally absent in these engineered constructs. Therefore, in an attempt to instill these hallmark properties of cartilage in engineered cell-seeded constructs, we designed and characterized a novel sliding contact bioreactor to recapitulate the mechanical stimuli arising from physiologic joint loading (two contacting cartilage layers). Finite element modeling of this bioreactor system showed that tensile strains were direction-dependent, while both tensile strains and fluid motion were depth-dependent and highest in the region closest to the contact surface. Short-term sliding contact of mesenchymal stem cell (MSC)-seeded agarose improved chondrogenic gene expression in a manner dependent on both the axial strain applied and transforming growth factor-β supplementation. Using the optimized loading parameters derived from these short-term studies, long-term sliding contact was applied to MSC-seeded agarose constructs for 21 d. After 21 d, sliding contact significantly improved the tensile properties of MSC-seeded constructs and elicited alterations in type II collagen and proteoglycan accumulation as a function of depth; staining for these matrix molecules showed intense localization in the surface regions. These findings point to the potential of sliding contact to produce engineered cartilage constructs that begin to recapitulate the complex mechanical features of the native tissue.
Authors:
A H Huang; B M Baker; G A Ateshian; R L Mauck
Publication Detail:
Type:  Journal Article     Date:  2012-07-12
Journal Detail:
Title:  European cells & materials     Volume:  24     ISSN:  1473-2262     ISO Abbreviation:  Eur Cell Mater     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973416     Medline TA:  Eur Cell Mater     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  29-45     Citation Subset:  IM    
Affiliation:
McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, 36th Street and Hamilton Walk, Philadelphia, PA 19104, USA.lemauck@mail.med.upenn.edu.
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