Document Detail

Slicing embryos gently with laser light sheets.
MedLine Citation:
PMID:  22396246     Owner:  NLM     Status:  Publisher    
Light sheet microscopy is an easy to implement and extremely powerful alternative to established fluorescence imaging techniques such as laser scanning confocal, multi-photon and spinning disk microscopy. By illuminating the sample only with a thin slice of light, photo-bleaching is reduced to a minimum, making light sheet microscopy ideal for non-destructive imaging of fragile samples over extended periods of time. Millimeter-sized samples can be imaged rapidly with high resolution and high depth penetration. A large variety of instruments have been developed and optimized for a number of different samples: Bessel beams form thin light sheets for single cells, and selective plane illumination microscopy (SPIM) offers multi-view acquisition to image entire embryos with isotropic resolution. This review explains how light sheet microscopy involves a conceptually new microscope design and how it changes modern imaging in biology.
Jan Huisken
Related Documents :
15474746 - Different behaviors of microbubbles in the liver: time-related quantitative analysis of...
2425396 - Analysis of imaging parameters, relaxation time (t1,t2), and relative proton density as...
8084236 - Off-resonance spin locking for mr imaging.
21123416 - Imaging neuropeptide release in the drosophila neuromuscular junction (nmj).
22974116 - Synthesis and evaluation of novel 18f labeled 2-pyridinyl benzoxazole and benzothiazole...
17667466 - Evaluation of hypoxemic patients with transesophageal echocardiography.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-6
Journal Detail:
Title:  BioEssays : news and reviews in molecular, cellular and developmental biology     Volume:  -     ISSN:  1521-1878     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510851     Medline TA:  Bioessays     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Effect of Exercise on Affective and Self-efficacy Responses in Older and Younger Women.
Next Document:  Depression, anxiety, and risk factor control in patients after hospitalization for coronary heart di...