| Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1. | |
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MedLine Citation:
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PMID: 16537485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used the Sleeping Beauty (SB) transposable element as a tool to probe transposon-host cell interactions in vertebrates. The Miz-1 transcription factor was identified as an interactor of the SB transposase in a yeast two-hybrid screen. Through its association with Miz-1, the SB transposase down-regulates cyclin D1 expression in human cells, as evidenced by differential gene expression analysis using microarray hybridization. Down-regulation of cyclin D1 results in a prolonged G(1) phase of the cell cycle and retarded growth of transposase-expressing cells. G(1) slowdown is associated with a decrease of cyclin D1/cdk4-specific phosphorylation of the retinoblastoma protein. Both cyclin D1 down-regulation and the G(1) slowdown induced by the transposase require Miz-1. A temporary G(1) arrest enhances transposition, suggesting that SB transposition is favored in the G(1) phase of the cell cycle, where the nonhomologous end-joining pathway of DNA repair is preferentially active. Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event. |
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Authors:
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Oliver Walisko; Zsuzsanna Izsvák; Kornélia Szabó; Christopher D Kaufman; Steffi Herold; Zoltán Ivics |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-07 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 103 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-15 Completed Date: 2006-04-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 4062-7 Citation Subset: IM |
Affiliation:
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Max Delbrück Center for Molecular Medicine, D-13092 Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Cycle / physiology* Cricetinae Cyclin D1 / metabolism DNA-Binding Proteins / chemistry, genetics, metabolism* Down-Regulation G1 Phase / physiology Hela Cells Humans Kruppel-Like Transcription Factors Oligonucleotide Array Sequence Analysis Phosphorylation Recombinant Fusion Proteins / chemistry, genetics, metabolism Retinoblastoma Protein / chemistry, metabolism Transcription Factors / chemistry, genetics, metabolism* Transposases / chemistry, genetics, metabolism* Two-Hybrid System Techniques Zinc Fingers |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Kruppel-Like Transcription Factors; 0/Recombinant Fusion Proteins; 0/Retinoblastoma Protein; 0/Transcription Factors; 0/ZBTB17 protein, human; 136601-57-5/Cyclin D1; EC 2.7.7.-/Transposases; EC 2.7.7.-/sleeping beauty transposase, human |
| Comments/Corrections | |
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