Document Detail

Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1.
MedLine Citation:
PMID:  16537485     Owner:  NLM     Status:  MEDLINE    
We used the Sleeping Beauty (SB) transposable element as a tool to probe transposon-host cell interactions in vertebrates. The Miz-1 transcription factor was identified as an interactor of the SB transposase in a yeast two-hybrid screen. Through its association with Miz-1, the SB transposase down-regulates cyclin D1 expression in human cells, as evidenced by differential gene expression analysis using microarray hybridization. Down-regulation of cyclin D1 results in a prolonged G(1) phase of the cell cycle and retarded growth of transposase-expressing cells. G(1) slowdown is associated with a decrease of cyclin D1/cdk4-specific phosphorylation of the retinoblastoma protein. Both cyclin D1 down-regulation and the G(1) slowdown induced by the transposase require Miz-1. A temporary G(1) arrest enhances transposition, suggesting that SB transposition is favored in the G(1) phase of the cell cycle, where the nonhomologous end-joining pathway of DNA repair is preferentially active. Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event.
Oliver Walisko; Zsuzsanna Izsvák; Kornélia Szabó; Christopher D Kaufman; Steffi Herold; Zoltán Ivics
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  103     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-15     Completed Date:  2006-04-25     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4062-7     Citation Subset:  IM    
Max Delbrück Center for Molecular Medicine, D-13092 Berlin, Germany.
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MeSH Terms
CHO Cells
Cell Cycle / physiology*
Cyclin D1 / metabolism
DNA-Binding Proteins / chemistry,  genetics,  metabolism*
G1 Phase / physiology
HeLa Cells
Kruppel-Like Transcription Factors
Oligonucleotide Array Sequence Analysis
Recombinant Fusion Proteins / chemistry,  genetics,  metabolism
Retinoblastoma Protein / chemistry,  metabolism
Transcription Factors / chemistry,  genetics,  metabolism*
Transposases / chemistry,  genetics,  metabolism*
Two-Hybrid System Techniques
Zinc Fingers
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Kruppel-Like Transcription Factors; 0/Recombinant Fusion Proteins; 0/Retinoblastoma Protein; 0/Transcription Factors; 0/ZBTB17 protein, human; 136601-57-5/Cyclin D1; EC 2.7.7.-/Transposases; EC 2.7.7.-/sleeping beauty transposase, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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