Document Detail

Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast.
MedLine Citation:
PMID:  21487389     Owner:  NLM     Status:  MEDLINE    
Genetic screening of yeast for sld (synthetic lethality with dpb11) mutations has identified replication proteins, including Sld2, -3, and -5, and clarified the molecular mechanisms underlying eukaryotic chromosomal DNA replication. Here, we report a new replication protein, Sld7, identified by rescreening of sld mutations. Throughout the cell cycle, Sld7 forms a complex with Sld3, which associates with replication origins in a complex with Cdc45, binds to Dpb11 when phosphorylated by cyclin-dependent kinase, and dissociates from origins once DNA replication starts. However, Sld7 does not move with the replication fork. Sld7 binds to the nonessential N-terminal portion of Sld3 and reduces its affinity for Cdc45, a component of the replication fork. Although Sld7 is not essential for cell growth, its absence reduces the level of cellular Sld3, delays the dissociation from origins of GINS, a component of the replication fork, and slows S-phase progression. These results suggest that Sld7 is required for the proper function of Sld3 at the initiation of DNA replication.
Tamon Tanaka; Toshiko Umemori; Shizuko Endo; Sachiko Muramatsu; Masato Kanemaki; Yoichiro Kamimura; Chikashi Obuse; Hiroyuki Araki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-12
Journal Detail:
Title:  The EMBO journal     Volume:  30     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-19     Completed Date:  2011-07-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  2019-30     Citation Subset:  IM    
Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, Shizuoka, Japan.
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MeSH Terms
Carrier Proteins / genetics,  metabolism*
Cell Cycle Proteins / metabolism*
Chromosomes, Fungal / metabolism*
DNA Mutational Analysis
DNA Replication*
DNA-Binding Proteins / metabolism*
Mutant Proteins / genetics,  metabolism
Nuclear Proteins / metabolism
Protein Binding
Protein Interaction Mapping
Saccharomyces cerevisiae / genetics,  growth & development,  physiology*
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Reg. No./Substance:
0/CDC45 protein, S cerevisiae; 0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/DPB11 protein, S cerevisiae; 0/Mutant Proteins; 0/Nuclear Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Sld3 protein, S cerevisiae; 0/Sld7 protein, S cerevisiae

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