| Skp2 inversely correlates with p27 and tuberin in transformed cells. | |
| | |
MedLine Citation:
|
PMID: 18604603 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cyclin-dependent kinase inhibitor p27Kip1 (p27) is a major gatekeeper of the mammalian cell cycle progression known to be regulated by both, its subcellular localization and its degradation. To allow entrance into S phase and thereby mammalian cell cycle progression p27 must be degraded by a skp2-containing E3 ubiquitin ligase whose task is to target p27 for degradation by the proteasome. The tumor suppressor gene product tuberin directly binds to p27 and protects it from degradation via skp2. Whereas, p27 and tuberin are known to be localized to both, the cytoplasm and the nucleus, the localization of skp2 remained elusive. Here we demonstrate that skp2 is a cytoplasmic and nuclear protein. In addition we found an inverse correlation of the endogenous protein levels of skp2 with p27 and tuberin in different transformed cells and under different growth conditions. These data allow new important insights into this molecular network of cell cycle control. |
| | |
Authors:
|
M Rosner; M Hanneder; N Siegel; A Valli; C Fuchs; M Hengstschl??ger |
Related Documents
:
|
2478343 - The role of a cell-associated hyaluronan-binding protein in fibroblast behaviour. 9809983 - Multinuclearity and increased ploidy caused by overexpression of the aurora- and ipl1-l... 2835303 - A morphometric study of liver cell dysplasia. |
Publication Detail:
|
Type: Journal Article Date: 2008-07-06 |
Journal Detail:
|
Title: Amino acids Volume: 37 ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-07-06 Completed Date: 2009-12-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
|
Languages: eng Pagination: 257-62 Citation Subset: IM |
Affiliation:
|
Medical Genetics, Medical University of Vienna, W??hringer G??rtel 18-20, 1090 Vienna, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Cell Line, Tumor Cell Nucleus / metabolism Cell Transformation, Neoplastic Cyclin-Dependent Kinase Inhibitor p27 / genetics, metabolism* Cytoplasm / metabolism Humans Rats S-Phase Kinase-Associated Proteins / genetics, metabolism* Tumor Suppressor Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/S-Phase Kinase-Associated Proteins; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 169027-60-5/tuberous sclerosis complex 2 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The methyltransferase domain of the 1a protein of cowpea chlorotic mottle virus controls local and s...
Next Document: Lack of repeatable differential expression patterns between MON810 and comparable commercial varieti...