Document Detail

Skin lipid profiling in normal and seborrhoeic shih tzu dogs.
MedLine Citation:
PMID:  23331684     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Seborrhoea is a clinical condition resulting in excessive lipid and/or scale on the skin and is a common and important skin disease of dogs. However, there is little information on the skin surface lipid composition of dogs with seborrhoea.
HYPOTHESIS/OBJECTIVES: To compare skin surface lipid profiles in normal and seborrhoeic shih tzu dogs.
METHODS: Fourteen client-owned dogs (seven seborrhoeic and seven normal) were investigated. Lipids in sebaceous glands (SGs) were extracted from homogenized tissues of SG hyperplasia. Surface lipid was collected by tape stripping [stratum corneum (SC)-enriched fraction] and acetone-wetted cotton swab (acetone-extracted fraction). Lipids in SGs, SC-enriched fractions and acetone-extracted fractions were evaluated by high-performance thin-layer chromatography.
RESULTS: Lipids in SGs mainly consisted of cholesterol esters, wax esters and triglycerides, whereas lipids in the SC-enriched fraction mainly consisted of ceramides. The acetone-extracted fraction contained a mixture of lipid classes recognized in SG- and SC-enriched fractions. In seborrhoeic dogs, concentrations of wax esters and triglycerides in the acetone-extracted fraction were significantly higher than in control dogs (P = 0.0285). Amounts of total ceramides (in micrograms) per milligram of SC were not significantly different between the two groups (P = 0.5204). Interestingly, two unknown ceramide fractions, which accounted for 20% of the total ceramides, were recognized exclusively in seborrhoeic dogs.
CONCLUSIONS AND CLINICAL IMPORTANCE: These results provide evidence that the skin surface lipid profiles are altered in shih tzu dogs with seborrhoea.
Ji-Seon Yoon; Koji Nishifuji; Sinpei Ishioroshi; Kaori Ide; Toshiroh Iwasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary dermatology     Volume:  24     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-21     Completed Date:  2013-07-03     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  84-9.e21-2     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD.
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MeSH Terms
Case-Control Studies
Dermatitis, Seborrheic / metabolism,  veterinary*
Dog Diseases / metabolism*
Epidermis / metabolism
Lipid Metabolism / physiology*
Skin / metabolism*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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