Document Detail


Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease.
MedLine Citation:
PMID:  23039882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in phenotype and function of γδ T cells have been reported in inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC). Dysregulation of lymphocyte migration plays a key role in IBD pathogenesis; however, data on migratory properties of γδ T cells are scarce. Human circulating γδ T cells from healthy controls (n = 27), patients with active CD (n = 15), active UC (n = 14) or cutaneous manifestations of IBD (n = 2) were characterized by flow cytometry. Circulating γδ T cells in healthy controls were CD3(hi) and expressed CD45RO. They expressed gut-homing molecule β7 but not gut-homing molecule corresponding chemokine receptors (CCR)9, or skin-homing molecules cutaneous lymphocyte-associated antigen (CLA) and CCR4, despite conventional T cells containing populations expressing these molecules. CCR9 expression was increased on γδ T cells in CD and UC, while skin-homing CLA was expressed aberrantly on γδ T cells in patients with cutaneous manifestations of IBD. Lower levels of CD3 expression were found on γδ T cells in CD but not in UC, and a lower proportion of γδ T cells expressed CD45RO in CD and UC. Enhanced expression of gut-homing molecules on circulating γδ T cells in IBD and skin-homing molecules in cutaneous manifestations of IBD may be of clinical relevance.
Authors:
E R Mann; N E McCarthy; S T C Peake; A N Milestone; H O Al-Hassi; D Bernardo; C T Tee; J Landy; M C Pitcher; S A Cochrane; A L Hart; A J Stagg; S C Knight
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  170     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-03-18     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  122-30     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Clinical and Experimental Immunology © 2012 British Society for Immunology.
Affiliation:
Antigen Presentation Research Group, Imperial College London, Northwick Park and St Mark’s Campus, Level 7W, St Mark’s Hospital,Watford Road, Harrow HA1 3UJ, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD3 / immunology,  metabolism
Antigens, CD45 / immunology,  metabolism
Antigens, Differentiation, T-Lymphocyte / immunology,  metabolism
Colitis, Ulcerative / immunology,  metabolism
Crohn Disease / immunology,  metabolism*
Female
Gastrointestinal Tract / immunology*,  metabolism
Humans
Inflammatory Bowel Diseases / immunology*,  metabolism
Integrin beta Chains / immunology,  metabolism
Male
Membrane Glycoproteins / immunology,  metabolism
Receptors, CCR / immunology,  metabolism
Receptors, CCR4 / immunology,  metabolism
Skin / immunology*,  metabolism
T-Lymphocyte Subsets / immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/Antigens, Differentiation, T-Lymphocyte; 0/CC chemokine receptor 9; 0/CCR4 protein, human; 0/CTAGE1 protein, human; 0/Integrin beta Chains; 0/Membrane Glycoproteins; 0/Receptors, CCR; 0/Receptors, CCR4; 0/integrin beta7; EC 3.1.3.48/Antigens, CD45
Comments/Corrections

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