Document Detail


Skin facilitates Candida albicans mating.
MedLine Citation:
PMID:  12933839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mating between natural a/a and alpha/alpha strains of Candida albicans requires that cells first switch from the white to opaque phase phenotype. However, because cells expressing the opaque phase phenotype are induced to switch back to the white phase phenotype at physiological temperature (37 degrees C) and because opaque phase cells are highly efficient at colonizing skin, we tested whether skin, which is several degrees lower than physiological temperature, is conducive to mating. Using a model in which a mixture of a/a and alpha/alpha cells are incubated for 24 h under a cotton patch on the hairless skin of newborn mice and using scanning electron microscopy to visualize cells on skin, it was demonstrated that skin facilitates mating. In some regions of the skin, 40% of all cells had fused. All of the stages of mating observed in vitro were observed in vivo. However, some unique morphological characteristics of mating on skin were observed and are attributable to parent cell immobilization on the skin. In control experiments on glass, plastic, and silicone elastomer surfaces at 32 degrees C, cells either failed to fuse or did so at an extremely low frequency, suggesting that unique features of the skin surface other than reduced temperature also facilitate fusion.
Authors:
Salil A Lachke; Shawn R Lockhart; Karla J Daniels; David R Soll
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  71     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-22     Completed Date:  2003-09-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4970-6     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Candida albicans / genetics,  physiology*,  ultrastructure
Genes, Fungal
Mice
Microscopy, Electron, Scanning
Phenotype
Skin / microbiology*
Skin Temperature
Surface Properties
Grant Support
ID/Acronym/Agency:
AI2392/AI/NIAID NIH HHS
Comments/Corrections

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