Document Detail

Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training.
MedLine Citation:
PMID:  18716035     Owner:  NLM     Status:  MEDLINE    
Pyruvate dehydrogenase (PDH) plays an important role in regulating carbohydrate metabolism in skeletal muscle. PDH is activated by PDH phosphatase (PDP) and deactivated by PDH kinase (PDK). Obesity has a large negative impact on skeletal muscle carbohydrate metabolism, whereas endurance training has been shown to improve regulatory control of skeletal muscle carbohydrate metabolism, more so when coupled with obesity. A majority of this literature has focused on PDK, with little information available on PDP. To determine the relative role of PDP in regulating skeletal muscle PDH activity with obesity and endurance training, obese and lean Zucker rats remained sedentary or were endurance trained (1 h/day, 5 days/wk) for a period of 8 wk. Soleus, red gastrocnemius, (RG), and white gastrocnemius (WG) muscles were sampled after the training period. The main findings were 1) obesity resulted in a 46% decrease in PDP activity expressed per milligram extracted mitochondrial protein only in RG, while PDP isoform content was unchanged; 2) 8 wk of endurance training led to a significant 1.4-2.2-fold increase in PDP activity of all muscle examined from obese rats, and the concomitant increase in PDP1 protein was only seen in soleus and RG; 3) 8 wk of endurance training led to a trending 1.4-2.2-fold increase in PDP activity of all muscle examined from obese rats, and the concomitant increase in PDP1 protein was only seen in soleus and RG; and 4) PDP2 protein content was not affected by obesity or training. These results suggest that decreased PDP activity in oxidative skeletal muscles may play a role in the impairment of carbohydrate metabolism in obese rats, which is reversible with endurance training.
Paul J Leblanc; Matthew Mulligan; Anamaria Antolic; Laura Macpherson; J Greig Inglis; Dale Martin; Brian D Roy; Sandra J Peters
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-20
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  295     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-03     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1224-30     Citation Subset:  IM    
Centre for Muscle Metabolism and Biophysics and Faculty of Applied Health Sciences, Brock University, St. Catharines, Ontario, Canada.
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MeSH Terms
Blood Glucose / metabolism
Body Weight / physiology
Citrate (si)-Synthase / metabolism
Fatty Acids, Nonesterified / blood
Insulin / blood
Isoenzymes / metabolism
Mitochondria / enzymology
Muscle, Skeletal / enzymology*
Obesity / blood,  physiopathology*
Physical Conditioning, Animal / physiology*
Pyruvate Dehydrogenase (Lipoamide) / metabolism
Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / metabolism*
Rats, Zucker
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids, Nonesterified; 0/Isoenzymes; 11061-68-0/Insulin; EC Dehydrogenase (Lipoamide); EC dehydrogenase E1alpha subunit; EC (si)-Synthase; EC Dehydrogenase (Lipoamide)-Phosphatase

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