Document Detail

Skeletal muscle interleukin-6 and tumor necrosis factor-alpha release in healthy subjects and patients with type 2 diabetes at rest and during exercise.
MedLine Citation:
PMID:  12870175     Owner:  NLM     Status:  MEDLINE    
To examine the influence of type 2 diabetes on cytokine release from the leg at rest and during exercise, 9 male type 2 diabetics (D) and 8 age-, gender-, Vo2peak-, weight- and body mass index (BMI)-matched control subjects (C) were studied before and after 25 minutes of supine bicycle exercise at 60% Vo2peak. Blood samples were obtained from a femoral artery and vein from 1 limb, and plasma was analyzed for glucose and the cytokines, interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha. Leg blood flow (LBF) was measured by thermodilution in the femoral vein, and net leg IL-6, TNF-alpha, and glucose balance were calculated as the product of LBF and femoral arteriovenous (fa-v) glucose, IL-6, and TNF-alpha difference. Arterial plasma glucose and IL-6 were higher (P<.05) at rest in D compared with C, but there were no differences in arterial TNF-alpha concentrations at rest when comparing groups. Despite measurable arterial levels of both IL-6 and TNF-alpha in both groups at rest, there was not net leg release of either cytokine at rest. Exercise increased (P<.05) IL-6 release and glucose uptake in both D and C, and contracting leg glucose uptake was similar when comparing D with C. While not significant, there was a trend (P=.1) for augmented exercise-induced IL-6 release in D compared with C. In contrast, exercise did not result in TNF-alpha release in either D or C. These data demonstrate that basal circulating TNF-alpha is not elevated in patients with type 2 diabetes when matched for BMI with control subjects. The results also suggest that neither type 2 diabetic nor healthy skeletal muscle releases these cytokines at rest, indicating that organs other than skeletal muscle contribute to the elevated basal IL-6 in type 2 diabetics. In contrast with IL-6, exercise does not result in the release of TNF-alpha from the contracting limbs of either healthy subjects or patients with type 2 diabetes.
Mark A Febbraio; Adam Steensberg; Rebecca L Starkie; Glenn K McConell; Bronwyn A Kingwell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  52     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-18     Completed Date:  2003-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  United States    
Other Details:
Languages:  eng     Pagination:  939-44     Citation Subset:  IM    
Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia.
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MeSH Terms
Blood Flow Velocity
Blood Glucose / analysis
Body Mass Index
Diabetes Mellitus, Type 2 / physiopathology*
Femoral Artery
Interleukin-6 / blood,  secretion*
Middle Aged
Muscle, Skeletal / secretion*
Oxygen Consumption
Tumor Necrosis Factor-alpha / secretion*
Reg. No./Substance:
0/Blood Glucose; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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