| Skeletal examination by double staining for ossified bone and cartilaginous tissue. | |
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MedLine Citation:
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PMID: 23138907 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The assessment of developmental toxicology data is a critical aspect of hazard evaluation for pharmaceuticals and environmental chemicals. Skeletal examination is an essential part of prenatal developmental toxicity studies of chemicals as well as pesticides and comprises evaluation of both cartilaginous and ossified skeletal components. Various techniques are published in the literature to process and double-stain skeletons of common laboratory animals which are all based on staining of the cartilage with Alcian Blue and staining of ossified bones with Alizarin Red S along with maceration (clearing) of the surrounding soft tissue. The staining of the cartilage allows the examination to assess ossified structures and their underlying cartilage in a single step. |
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Authors:
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Steffen Schneider |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 947 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 215-21 Citation Subset: IM |
Affiliation:
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BASF SE, Ludwigshafen, Germany, steffen.schneider@basf.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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